Transcriptional and posttranscriptional regulation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase during liver regeneration.
Transcriptional and posttranscriptional regulation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase during liver regeneration.
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DOI：
10.1073/pnas.89.9.3746
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发表时间：
1992-05
影响因子：
11.1
通讯作者：
J. L. Rosa;A. Tauler;A. Lange;S. Pilkis;R. Bartrons
中科院分区：
文献类型：
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作者：
J. L. Rosa;A. Tauler;A. Lange;S. Pilkis;R. Bartrons
研究了肝再生过程中6-磷酸果糖-2-激酶/果糖-2，6-二磷酸酶（PFK-2/FBPase-2; EC 2.7.1.105/3.1.3.46）基因表达的控制。部分肝切除后6小时PFK-2/FBPase-2 mRNA水平降至对照值的5%左右。此后的mRNA增加到最大值，在48小时，并恢复到正常水平，由96 hr. In假手术的动物，只有一个小的增加，观察到在第一个4 hr. The mRNA被识别的299碱基对的肝脏特异性cDNA探针，但不是由肌肉特异性探针。mRNA调节的时间过程与PFK-2/FBPase-2活性和双功能酶蛋白的量具有良好的相关性，所述双功能酶蛋白的量通过用针对肝脏PFK-2/FBPase-2的N-末端十肽的抗体进行免疫印迹来确定。部分肝切除后PFK-2/FBPase-2 mRNA的降解率无明显变化。肝再生过程中PFK-2/FBPase-2基因表达的调节涉及转录速率的变化。肝切除术后6小时，该比率下降了50%。此后的速率增加到最大值在72小时，然后返回到控制值由96 hr. The白蛋白的转录速率没有改变，而磷酸烯醇式丙酮酸羧激酶增加12倍，在6 hr. These结果表明，PFK-2/FBPase-2基因转录的具体调控，这种调节是在部分负责在再生肝脏中看到的肝代谢的改变。
The control of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2; EC 2.7.1.105/3.1.3.46) gene expression during liver regeneration was studied. The level of PFK-2/FBPase-2 mRNA decreased to about 5% of the control value 6 hr after partial hepatectomy. Thereafter the mRNA increased to a maximum at 48 hr and returned to normal levels by 96 hr. In sham-operated animals, only a small increase was observed during the first 4 hr. The mRNA was recognized by a 299-base-pair liver-specific cDNA probe but not by a muscle-specific probe. The time course of mRNA modulation was well correlated with PFK-2/FBPase-2 activity and with the amount of bifunctional enzyme protein determined by immunoblotting with an antibody raised against the N-terminal decapeptide of liver PFK-2/FBPase-2. No alteration in the degradation rate of PFK-2/FBPase-2 mRNA was noted after partial hepatectomy. The modulation of PFK-2/FBPase-2 gene expression during liver regeneration involved changes in the transcription rate. The rate decreased by 50% at 6 hr after liver resection. The rate increased thereafter to a maximum at 72 hr and then returned to control values by 96 hr. The transcription rate of albumin did not change, whereas that of phosphoenolpyruvate carboxykinase increased 12-fold at 6 hr. These results show that PFK-2/FBPase-2 gene transcription is specifically regulated and that this regulation is in part responsible for the alterations in hepatic metabolism seen in regenerating liver.