Identification and characterisation of ten glutathione S-transferase genes from oriental migratory locust, Locusta migratoria manilensis (Meyen)
Identification and characterisation of ten glutathione S-transferase genes from oriental migratory locust, Locusta migratoria manilensis (Meyen)
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东方飞蝗 Locusta migratoria manilensis (Meyen) 10 个谷胱甘肽 S-转移酶基因的鉴定和表征
DOI：
10.1002/ps.2110
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发表时间：
2011-06-01
影响因子：
4.1
通讯作者：
Zhang, Jianzhen
中科院分区：
文献类型：
--
作者：
Qin, Guohua;Jia, Miao;Zhang, Jianzhen
背景：合成拟除虫菊酯是广泛用于控制马尼拉飞蝗（Meyen）的主要杀虫剂，马尼拉飞蝗是东亚、南亚和太平洋地区的主要害虫。本文对马尼拉 L. migratoria manilensis 中 10 个编码谷胱甘肽 S-转移酶 (GST) 的 cDNA 进行了测序和表征。研究了溴氰菊酯对 10 个 GST 基因表达的影响。结果：系统发育分析发现 9 个 GST 属于 3 个不同的类别，其中 7 个属于 Sigma，1 个属于 Delta，1 个属于 θ。剩余的 GST (LmGSTu1) 未分类。 RT-PCR分析显示，除LmGSTs2、LmGSTs4、LmGSTs7和LmGSTu1在少数组织中表达外，大多数GST基因在所有检查的组织中均有表达，包括前肠、中肠、盲肠、后肠、马氏小管、脂肪体、肌肉、睾丸和卵巢。 LmGSTu1 似乎是唯一无法在鸡蛋中检测到表达的基因。实时定量PCR表明，0.08和/或0.12μg·mL(-1)溴氰菊酯几乎增加了三龄若虫蝗虫中所有10个GST基因的表达。然而，0.16和/或0.2 mu g mL(-1)溴氰菊酯可降低LmGSTd1、LmGSTs1、LmGSTs5和LmGSTs6的表达。结论：马尼拉飞蝗接触溴氰菊酯后GST基因表达增加，可能导致其对其他杀虫剂和异生物质的耐受性升高。 (C) 2011年化学工业学会
BACKGROUND: Synthetic pyrethroids are the primary insecticides that are widely used for controlling Locusta migratoria manilensis (Meyen), a major pest in eastern and southern Asia and the Pacific region. In this paper, ten cDNAs encoding glutathione S-transferases (GSTs) were sequenced and characterised in L. migratoria manilensis. The effects of deltamethrin on the ten GST gene expressions were studied.RESULTS: Phylogenetic analysis revealed nine GSTs in three different classes, including seven in sigma, one in delta and one in theta. The remaining GST (LmGSTu1) was unclassified. RT-PCR analysis showed that most GST genes were expressed in all tissues examined, including the foregut, midgut, gastric caecum, hindgut, Malpighian tubules, fat bodies, muscles, spermaries and ovaries, except that LmGSTs2, LmGSTs4, LmGSTs7 and LmGSTu1 were expressed in several tissues. LmGSTu1 appeared to be the only gene whose expressions could not be detected in eggs. Real-time quantitative PCR showed that deltamethrin at 0.08 and/or 0.12 mu g mL(-1) increased almost all ten GST gene expressions in third-instar nymph locusts. However, deltamethrin at 0.16 and/or 0.2 mu g mL(-1) decreased the expressions of LmGSTd1, LmGSTs1, LmGSTs5 and LmGSTs6.CONCLUSION: The increases in GST gene expressions after deltamethrin exposure in L. migratoria manilensis might result in its elevating tolerance to other insecticides and xenobiotics. (C) 2011 Society of Chemical Industry