Expression of a human endogenous retrovirus, HERV-K, in the blood cells of leukemia patients
Expression of a human endogenous retrovirus, HERV-K, in the blood cells of leukemia patients
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DOI：
10.1038/sj.leu.2402355
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发表时间：
2002-02-01
期刊：
影响因子：
11.4
通讯作者：
Prin, L
中科院分区：
文献类型：
--
作者：
Depil, S;Roche, C;Prin, L
人类内源性逆转录病毒序列（HERV）被认为是致癌过程中可能的致病因子。 HERV-K 是最具生物活性的形式，因为该家族的成员具有完整的 gag、pol 或 env 基因开放阅读框。据报道，白血病患者出现了针对 HERV-K 肽的抗体反应，这表明该序列在白血病细胞中可能过度表达。使用实时定量 RT-PCR (TaqMan)，我们发现在我们收集的 8 个白血病样本中的 6 个中，HERV-K10 样 gag 基因的转录活性比正常外周血单核细胞 (PBMC) 或脐带血单核细胞高 5 至 10 倍。过度表达的标记足以通过 Northern blot 检测到。此外，在暴露于可能调节 HERV 表达的不同因素（PHA、伽马射线照射、5-氮杂胞苷）后，正常 PBMC 中的 HERV-K 表达没有显着变化。这表明白血病样本中 HERV-K 的相对过度表达可能与肿瘤的发展特定相关。因此，这些转录变异的起源值得进一步研究。
Human endogenous retroviral sequences (HERVs) are believed to be possible pathogenic agents in carcinogenesis. HERV-K is the most biologically active form, since members of this family have intact open reading frames for the gag, pol or env genes. Antibody response against HERV-K peptides has been reported in leukemia patients, suggesting a possible overexpression of this sequence in leukemic cells. Using real-time quantitative RT-PCR (TaqMan), we found that in six of the eight leukemia samples we collected, transcriptional activity of HERV-K10-like gag gene was 5- to 10-fold higher than in normal peripheral blood mononuclear cells (PBMCs) or mononuclear cells from cord blood. The overexpression was marked enough to be detected by Northern blot. In addition, there was no significant variation of HERV-K expression in normal PBMCs after exposure to different factors (PHA, gamma irradiation, 5-azacytidine) that potentially modulate HERV expression. This suggests that HERV-K relative overexpression in leukemia samples might be specifically associated with tumor development. The origin of these transcriptional variations is therefore worth being investigated further.