MUTAGENICITY OF HALOGENATED ALIPHATIC-HYDROCARBONS IN SALMONELLA-TYPHIMURIUM, STREPTOMYCES-COELICOLOR AND ASPERGILLUS-NIDULANS
MUTAGENICITY OF HALOGENATED ALIPHATIC-HYDROCARBONS IN SALMONELLA-TYPHIMURIUM, STREPTOMYCES-COELICOLOR AND ASPERGILLUS-NIDULANS
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DOI：
10.1016/0009-2797(80)90110-6
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发表时间：
1980-01-01
影响因子：
5.1
通讯作者：
CARERE, A
中科院分区：
文献类型：
--
作者：
BIGNAMI, M;CONTI, G;CARERE, A
测试了8种结构上相关的卤代脂肪烃（一、二和三氯乙醛（最后一种为无水和水合物形式）、一、二和三氯乙醇和烯丙基氯）诱导原核和真核微生物基因突变的能力。采用的遗传系统是S.使用菌株TA 1535和TA 100进行的鼠伤寒回复突变试验（有和无[哺乳动物]代谢活化）、S. coelicolor和2个正向突变系统。nidulans。用斑点和平板掺入测定技术测试每种化合物。在所有检测的微生物中，一、二和三氯乙醛均具有致突变性。卤代乙醇在A. nidulans，而S. typhimurium和S.唯一的活性形式分别是单氯衍生物和二氯衍生物。烯丙基氯在S.鼠伤寒沙门氏菌和S. coelicolor和A. nidulans。技术方法和不同因素（毒性、挥发性和稳定性）对每种供试化合物遗传反应的复杂影响，只允许在致突变效力和化学结构之间建立定性关系。
Eight structurally related halogenated aliphatic hydrocarbons (mono-, di- and trichloroacetaldehyde (the last in the anhydrous and hydrate form), mono-, di- and trichloroethanol and allyl chloride) were tested for their ability to induce gene mutations in prokaryotic and eukaryotic microorganisms. The genetic systems employed were the S. typhimurium reversion test with strain TA 1535 and TA 100, with and without [mammalian] metabolic activation, a forward and a back-mutation system in S. coelicolor and 2 forward mutation systems in A. nidulans. Each compound was tested with the spot and plate incorporation assay techniques. Mono-, di- and trichloroacetaldehyde were mutagenic in all the microorganisms tested. The halogenated ethanols were positive in A. nidulans, while in S. typhimurium and in S. coelicolor the only active forms were the mono- and dichloroderivatives, respectively. Allyl chloride was active in S. typhimurium and S. coelicolor and negative in A. nidulans. The technical approach and the complex influence of different factors (toxicity, volatility and stability) on the genetic response of each of the compounds under test did not allow more than a qualitative relationship between mutagenic potency and chemical structure.