The Mediterranean diet decreases prothrombotic microvesicle release in asymptomatic individuals at high cardiovascular risk
The Mediterranean diet decreases prothrombotic microvesicle release in asymptomatic individuals at high cardiovascular risk
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DOI：
10.1016/j.clnu.2020.02.027
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发表时间：
2020-11-01
影响因子：
6.3
通讯作者：
Badimon, Lina
中科院分区：
文献类型：
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作者：
Chiva-Blanch, Gemma;Sala-Vila, Aleix;Badimon, Lina
背景和目标：循环微泡（cMV）是小的富含磷脂的囊泡，有助于动脉粥样硬化血栓形成过程，是心血管疾病（CVD）负担和进展的生物标志物。饮食是CVD预防的基石，但饮食对cMV脱落的影响特征不明显。我们旨在评估地中海饮食与低脂饮食（LFD）相比对按照指南治疗的心血管高危患者的血液和血管室细胞MV脱落的长期影响。方法：PREDIMED试验中共有155名受试者在平均随访5年后未发生心血管事件研究中包括了来自地中海饮食补充特级初榨橄榄油-EVOO-的n = 53;来自地中海饮食补充混合坚果-坚果-的n = 49;和来自LFD的n = 53）。在基线和一年干预后，通过流式细胞术对cMV进行定量和表征，以通过膜联蛋白V（AV）结合来鉴定其活化的亲本细胞来源和促血栓形成潜力。经过一年的饮食干预，与LFD和EVOO干预相比，Nuts组血小板源性PAC-1（+）/AV（+）和CD 62 P（+）/AV（+）cMV浓度较低（P分别为0.036和0.003）。此外，与一年LFD相比，两种地中海饮食（EVOO和Nuts）干预中携带组织因子的血栓前cMV（CD 142（+）/AV（+））和源自活化细胞的CD 11 a（+）/AV（+）cMV显著降低（分别为P < 0.0001和0.028）。干预一年后，LFD组的SMA α（+）/AV（+）cMV低于Nuts组（P = 0.038）。结论：cMV是细胞活化和血管损伤的标志物，对饮食变化敏感。遵循富含EVOO或坚果的地中海饮食与朝向促动脉粥样硬化血栓形成表型的较低细胞活化相关，表明CV并发症的发展延迟。(C)2020爱思唯尔有限公司和欧洲临床营养与代谢学会。All rights reserved.
Background & aims: Circulating microvesicles (cMV) are small phospholipid-rich vesicles that contribute to the atherothrombotic process, and are biomarkers of cardiovascular disease (CVD) burden and progression. Diet is a cornerstone for CVD prevention, but dietary effects on cMV shedding are poorly characterized. We aimed at assessing the long term effects of a Mediterranean diet compared to a low-fat diet (LFD) on MV shedding by cells of the blood and vascular compartments in patients at high cardiovascular risk treated as per guidelines.Methods: A total of 155 participants from the PREDIMED trial free of cardiovascular events after a mean follow-up of 5 years (n = 53 from the Mediterranean diet supplemented with extra-virgin olive oil-EVOO-; n = 49 from the Mediterranean diet supplemented with mixed nuts -Nuts-; and n = 53 from the LFD) were included in the study. At baseline and after one-year intervention, cMV were quantified and characterized by flow cytometry to identify their activated parental cell origin and prothrombotic potential by Annexin V (AV) binding.Results: After one year of dietary intervention, platelet-derived PAC-1(+)/AV(+) and CD62P(+)/AV(+) cMV concentrations were lower in the Nuts group compared with the LFD and EVOO interventions (P = 0.036 and 0.003, respectively). In addition, prothrombotic cMV carrying tissue factor (CD142(+)/AV(+)) and CD11a(+) /AV(+) cMV derived from activated cells, were significantly lower in both Mediterranean diet (EVOO and Nuts) interventions compared to one year of LFD (P < 0.0001 and 0.028, respectively). SMA alpha(+)/AV(+) cMV were lower in the LFD compared to the Nuts group after one year of intervention (P = 0.038).Conclusions: cMV are markers of cell activation and vascular injury that appear to be sensitive to dietary changes. Following a Mediterranean diet rich in EVOO or nuts is associated with lower cell activation towards a pro-atherothrombotic phenotype, suggesting a delay in the development of CV complications. (C) 2020 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.