Ultrastructure of the basal lamina of bovine ovarian follicles and its relationship to the membrana granulosa
Ultrastructure of the basal lamina of bovine ovarian follicles and its relationship to the membrana granulosa
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DOI：
10.1530/reprod/118.2.221
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发表时间：
2000-03-01
期刊：
影响因子：
--
通讯作者：
Rodgers, RJ
中科院分区：
文献类型：
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作者：
Irving-Rodgers, HF;Rodgers, RJ
不同的形态表型的卵泡基膜和膜颗粒已被观察到。对10个直径<0.1mm的腔前卵泡、17个直径0.5 ~ 12 mm的健康卵泡和6个直径0.5 ~ 12 mm的闭锁卵泡进行光镜和电镜观察，以探讨卵泡基膜与颗粒膜的关系。在每个窦卵泡内，颗粒膜的基底细胞的形状是均匀的，圆形或柱状。直径≤ 4 mm的卵泡比例相等，基底细胞为柱状和圆形。较大的卵泡只有圆形的基底细胞。在窦前和窦期的健康卵泡中，观察到与基底颗粒细胞相邻的单层常规基底层。而在窦前期，传统类型的基膜增大甚至部分分层。第二种类型的基膜，被描述为“圈状”，发生在约一半的腔前卵泡和一半的腔卵泡小于或等于4 mm直径。在较大的卵泡中未观察到环状的基底层。“环状”基底层由基底层排列基底颗粒细胞的基底表面组成，但通常从最内层分支出额外的层或环。每个环通常< 1 μ m长，并有囊泡（20-30 nm）附着在内侧。基底细胞突起也很常见，可以看到小泡从这些突起中出芽。在有腔卵泡中，圆形基底颗粒细胞的卵泡出现常规基底层。其他卵泡与柱状细胞，闭锁卵泡，有'圈'基膜表型。因此，卵泡具有与颗粒膜形态相关的不同基底层。
Different morphological phenotypes of follicular basal lamina and of membrana granulosa have been observed. Ten preantral follicles (< 0.1 mm), and 17 healthy and six atretic antral follicles (0.5-12 mm in diameter) were processed for light and electron microscopy to investigate the relationship the between follicular basal lamina and membrana granulosa. Within each antral follicle, the shape of the basal cells of the membrana granulosa was uniform, and either rounded or columnar. There were equal proportions of follicles less than or equal to 4mm in diameter with columnar basal cells and with rounded basal cells. Larger follicles had only rounded basal cells. Conventional basal laminae of a single layer adjacent to the basal granulosa cells were observed in healthy follicles at the preantral and antral stages. However, at the preantral stage, the conventional types of basal lamina were enlarged or even partially laminated. A second type of basal lamina, described as 'loopy', occurred in about half the preantral follicles and in half the antral follicles less than or equal to 4 mm diameter. "Loopy' basal laminae were not observed in larger follicles. 'Loopy' basal laminae were composed of basal laminae aligning the basal surface of basal granulosa cells, but with additional layers or loops often branching from the innermost layer. Each loop was usually < 1 mu m long and had vesicles (20-30 nm) attached to the inner aspect. Basal cellular processes were also common, and vesicles could be seen budding off from these processes. In antral follicles, conventional basal laminae occurred in follicles with rounded basal granulosa cells. Other follicles with columnar cells, and atretic follicles, had the 'loopy' basal lamina phenotype. Thus, follicles have different basal laminae that relate to the morphology of the membrana granulosa.