Analyzing Spindle Positioning Dynamics in Cultured Cells
Analyzing Spindle Positioning Dynamics in Cultured Cells
复制标题
分析培养细胞中的主轴定位动力学
DOI：
10.1007/978-1-4939-3542-0_15
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发表时间：
2017
影响因子：
--
通讯作者：
Tomomi Kiyomitsu
中科院分区：
文献类型：
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作者：
杉木俊彦;藤原敏道;児嶋長次郎;杉木俊彦;Tomomi Kiyomitsu
由于有丝分裂过程中纺锤体的定位是一个高度动态的过程，活细胞成像是研究其潜在机制的关键技术。成像工具和显微镜系统的最新进展使我们能够以高的时间和空间分辨率同时可视化活细胞中的几种细胞成分。通过将活细胞成像与功能测定（例如基于RNAi的消耗、药物抑制和微图案化盖玻片）相结合，已经发现了纺锤体定位的新颖和意想不到的机制。在本章中，我提出了分析培养细胞中纺锤体定位动态的方法。
As spindle positioning during mitosis is a highly dynamic process, live cell imaging is a key technology when studying its underlying mechanisms. Recent advances in imaging tools and microscope systems have enabled us to simultaneously visualize several cellular components in living cells with high temporal and spatial resolution. By combining live cell imaging with functional assays such as RNAi-based depletions, drug inhibition, and micropatterned coverslips, novel and unexpected mechanisms of spindle positioning have been uncovered. In this chapter, I present methods for analyzing the dynamics of spindle positioning in cultured cells.