Atlas of Wnt and R-spondin gene expression in the developing male mouse lower urogenital tract.
Atlas of Wnt and R-spondin gene expression in the developing male mouse lower urogenital tract.
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DOI：
10.1002/dvdy.22741
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发表时间：
2011-11
影响因子：
2.5
通讯作者：
Vezina, Chad M.
中科院分区：
文献类型：
--
作者：
Mehta, Vatsal;Abler, Lisa L.;Keil, Kimberly P.;Schmitz, Christopher T.;Joshi, Pinak S.;Vezina, Chad M.
前列腺发育受β-连环蛋白信号传导的影响，但不清楚涉及哪些β-连环蛋白激活剂、它们在何处合成以及它们的mRNA丰度是否受雄激素影响。我们在转基因报告小鼠的下泌尿生殖道（LUT）中鉴定出对WNT/β -连环蛋白有反应的β -半乳糖苷酶活性，但在我们所检测的四种小鼠品系中，β -半乳糖苷酶活性存在差异。我们利用原位杂交比较了野生型胎鼠雄性、胎鼠雌性和新生雄性LUT中Wnts、R -脊椎蛋白（Rspos，β -连环蛋白信号传导的共激活剂）、β -连环蛋白反应性mRNAs以及雄激素受体反应性mRNA的模式。在前列腺发育过程中，大多数Wnt和Rspo mRNAs存在于LUT中。对于WNT/β -连环蛋白反应性基因以及Wnt2b、Wnt4、Wnt7a、Wnt9b、Wnt10b、Wnt11、Wnt16和Rspo3 mRNAs，观察到了性别二态性表达模式。这些结果揭示了胎鼠LUT中WNT/β -连环蛋白信号传导的性别差异，支持了该通路在前列腺导管发育过程中可能直接或间接对雄激素有反应的观点。
Prostate development is influenced by β-catenin signaling, but it is unclear which β-catenin activators are involved, where they are synthesized, and whether their mRNA abundance is influenced by androgens. We identified WNT/β-catenin-responsive β-galactosidase activity in the lower urogenital tract (LUT) of transgenic reporter mice, but β-galactosidase activity differed among the four mouse strains we examined. We used in situ hybridization to compare patterns of Wnts, r-spondins (Rspos, co-activators of β-catenin signaling), β-catenin-responsive mRNAs, and an androgen receptor-responsive mRNA in wild type fetal male, fetal female, and neonatal male LUT. Most Wnt and Rspo mRNAs were present in LUT during prostate development. Sexually dimorphic expression patterns were observed for WNT/β-catenin-responsive genes, and for Wnt2b, Wnt4, Wnt7a, Wnt9b, Wnt10b, Wnt11, Wnt16, and Rspo3 mRNAs. These results reveal sexual differences in WNT/β-catenin signaling in fetal LUT, supporting the idea that this pathway may be directly or indirectly responsive to androgens during prostate ductal development.
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影响因子：
2.5
作者：
Abler, Lisa L.;Keil, Kimberly P.;Mehta, Vatsal;Joshi, Pinak S.;Schmitz, Christopher T.;Vezina, Chad M.
通讯作者：
Vezina, Chad M.
影响因子：
4.6
作者：
Bell, Sheila M.;Schreiner, Claire M.;Whitsett, Jeffrey A.
通讯作者：
Whitsett, Jeffrey A.
影响因子：
4.8
作者：
Filali, M;Cheng, NL;Engelhardt, JF
通讯作者：
Engelhardt, JF
影响因子：
4.6
作者：
Kazanskaya, Olga;Ohkawara, Bisei;Niehrs, Christof
通讯作者：
Niehrs, Christof
影响因子：
2.9
作者：
Kuslak, Sheri L.;Marker, Paul C.
通讯作者：
Marker, Paul C.