Multi-purpose electrochemical biosensor based on a "green" homobifunctional cross-linker coupled with PAMAM dendrimer grafted p-MWCNTs as a platform: application to detect alpha 2,3-sialylated glycans and alpha 2,6-sialylated glycans in human serum
Multi-purpose electrochemical biosensor based on a "green" homobifunctional cross-linker coupled with PAMAM dendrimer grafted p-MWCNTs as a platform: application to detect alpha 2,3-sialylated glycans and alpha 2,6-sialylated glycans in human serum
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基于“绿色”同双功能交联剂与 PAMAM 树枝状聚合物接枝 p-MWCNT 为平台的多用途电化学生物传感器：用于检测人血清中的 α2,3-唾液酸化聚糖和 α2,6-唾液酸化聚糖
作者：
Niu Yazhen;He Junlin;Li Yuliang;Zhao Yilin;Xia Chunyong;Yuan Guolin;Zhang Lei;Zhang Yuchan;Yu Chao
唾液酸化聚糖是癌症诊断和临床研究的重要分子靶标。α 2，3-唾液酸化聚糖和α 2，6-唾液酸化聚糖是自然界中发现的主要唾液酸。糖链数量的不同表达可导致不同疾病的发生。然而，没有理想的方法来区分α 2，3-唾液酸化聚糖和α 2，6-唾液酸化聚糖。本工作制备了一种多功能的生物传感器，用于检测α 2，3-唾液酸化聚糖和α 2，6-唾液酸化聚糖。为了提高生物传感器的灵敏度，p-MWCNTs与PAMAM集成，因为PAMAM具有高度支化和丰富的氨基，提供了大的可用表面积用于与其他物质连接。为了实现可区分的识别，包括怀槐凝集素（MAL）和接骨木凝集素（SNA）。为了促进凝集素的固定，选择了一种绿色同双功能交联剂PDITC。在优化的检测条件下，α 2，3-唾液酸化聚糖的检测线性范围为10 fg mL−1至50 ng mL−1，检测下限为3 fg mL−1，α 2，6-唾液酸化聚糖的检测线性范围为10 fg mL−1至50 ng mL−1，检测下限为3 fg mL− 1。本研究不仅为α 2，3-唾液酸聚糖和α 2，6-唾液酸聚糖的鉴别检测提供了方法，也为今后的临床检测提供了参考。
Sialylated glycans are crucial molecular targets for cancer diagnosis and clinical research. α2,3-Sialylated glycans and α2,6-sialylated glycans are the predominant sialic acids found in nature. Different expression of the quantity of glycans can result in development of different disease. However, there are no ideal methods for discriminating α2,3-sialylated glycans and α2,6-sialylated glycans. In this work, a multi-purpose biosensor is fabricated for sensitive detection of α2,3-sialylated glycans and α2,6-sialylated glycans. To improve the sensitivity of the biosensor, p-MWCNTs were integrated with PAMAM, as PAMAM has highly branched and abundant amino groups, providing a large available surface area for linking with other substances. To achieve distinguishable recognition, Maackia amurensis lectin (MAL) and Sambucus nigra agglutinin (SNA) were included. To facilitate the lectin fixation, PDITC, a kind of green homobifunctional cross-linker, was selected. Under optimized detection conditions, the linear range of detection for α2,3-sialylated glycans is 10 fg mL−1 to 50 ng mL−1 with a lower detection limit of 3 fg mL−1, and the linear range of detection for α2,6-sialylated glycans is 10 fg mL−1 to 50 ng mL−1 with a detection limit of 3 fg mL−1. This work not only provides a method for distinguishing detection of α2,3-sialylated glycans and α2,6-sialylated glycans, but also provides a reference for future clinical testing.