Surface-Modified Bilosomes Nanogel Bearing a Natural Plant Alkaloid for Safe Management of Rheumatoid Arthritis Inflammation.
Surface-Modified Bilosomes Nanogel Bearing a Natural Plant Alkaloid for Safe Management of Rheumatoid Arthritis Inflammation.
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DOI：
10.3390/pharmaceutics14030563
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发表时间：
2022-03-03
期刊：
影响因子：
5.4
通讯作者：
Eid HM
中科院分区：
文献类型：
--
作者：
Elkomy MH;Alruwaili NK;Elmowafy M;Shalaby K;Zafar A;Ahmad N;Alsalahat I;Ghoneim MM;Eissa EM;Eid HM
风湿性关节炎（RA）是一种影响关节的慢性炎症性疾病。RA的特点是关节逐渐恶化。目前的RA治疗减轻了炎症和疼痛等体征，并大大减缓了疾病的进展。在这项研究中，我们的目的是促进小檗碱（一种天然产物）的透皮给药，通过将其封装在壳聚糖，表面改性的生物体纳米凝胶中，以更好地管理RA的炎症。根据薄膜水合方法和优化的各种因果变量，考虑到脂质，脱氧胆酸钠和壳聚糖浓度对颗粒大小，包埋和表面电荷的影响，壳聚糖包被的胆汁体与小檗碱（BER-CTS-BLS）进行了配制。优化后的BER-CTS-BLS平均粒径为202.3 nm，包封率为83.8%，表面电荷为30.8 mV。优化的BER-CTS-BLS在体外表现出延迟释放特性，并增加了离体皮肤渗透性。此外，组织学检查显示配制的BLS对皮肤没有刺激作用。此外，在具有角叉菜胶诱导的爪水肿的大鼠中评估优化的BER-CTS-BLS减轻炎症的能力。我们的结果表明，用局部BER-CTS-BLS凝胶处理的组显示出大鼠足水肿肿胀百分比的显著降低，在12小时后达到24.4%，这显著低于其他组。总的来说，含有小檗碱的壳聚糖包被的胆固醇体已经成为控制RA炎症的有希望的治疗方法。
Rheumatoid arthritis (RA) is a chronic inflammatory illness affecting the joints. The characteristic of RA is gradual joint deterioration. Current RA treatment alleviates signs such as inflammation and pain and substantially slows the progression of the disease. In this study, we aimed to boost the transdermal delivery of berberine (a natural product) by encapsulating it in chitosan, surface-modified bilosomes nanogel for better management of the inflammation of RA. The chitosan-coated bilosomes loaded with berberine (BER-CTS-BLS) were formulated according to the thin-film hydration approach and optimized for various causal variables, considering the effect of lipid, sodium deoxycholate, and chitosan concentrations on the size of the particles, entrapment, and the surface charge. The optimized BER-CTS-BLS has 202.3 nm mean diameter, 83.8% entrapment, and 30.8 mV surface charge. The optimized BER-CTS-BLS exhibited a delayed-release profile in vitro and increased skin permeability ex vivo. Additionally, histological examination revealed that the formulated BLS had no irritating effects on the skin. Furthermore, the optimized BER-CTS-BLS ability to reduce inflammation was evaluated in rats with carrageenan-induced paw edema. Our results demonstrate that the group treated with topical BER-CTS-BLS gel exhibited a dramatic reduction in rat paw edema swelling percentage to reach 24.4% after 12 h, which was substantially lower than other groups. Collectively, chitosan-coated bilosomes containing berberine have emerged as a promising therapeutic approach to control RA inflammation.
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DOI：
10.3390/ph15030281
发表时间：
2022-02-24
期刊：
Pharmaceuticals (Basel, Switzerland)
影响因子：
--
作者：
Abo El-Enin HA;Elkomy MH;Naguib IA;Ahmed MF;Alsaidan OA;Alsalahat I;Ghoneim MM;Eid HM
通讯作者：
Eid HM
影响因子：
3.4
作者：
Elkomy, Mohammed H.;El Menshawe, Shahira F.;Ali, Ahmed M. A.
通讯作者：
Ali, Ahmed M. A.
影响因子：
4.4
作者：
Ahmed, Tarek A.
通讯作者：
Ahmed, Tarek A.
影响因子：
5.4
作者：
El Menshawe, Shahira F.;Aboud, Heba M.;Abdeltwab, Amany M.
通讯作者：
Abdeltwab, Amany M.
影响因子：
6
作者：
Aboud, Heba M.;Ali, Adel Ahmed;Abd Elbary, Ahmed
通讯作者：
Abd Elbary, Ahmed