Expansion microscopy: principles and uses in biological research
Expansion microscopy: principles and uses in biological research
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DOI：
10.1038/s41592-018-0219-4
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发表时间：
2019-01-01
期刊：
影响因子：
48
通讯作者：
Boyden, Edward S.
中科院分区：
文献类型：
--
作者：
Wassie, Asmamaw T.;Zhao, Yongxin;Boyden, Edward S.
许多生物学研究需要具有纳米级空间分辨率的细胞或组织的3D成像。我们最近发现，保存的生物标本可以通过化学过程以各向同性的方式进行物理膨胀。扩展显微镜（ExM）允许用传统显微镜对生物标本进行纳米级成像，使生物分子减少以支持信号放大和多重读出化学，并使标本透明。我们回顾了ExM如何工作的原理，我们小组和其他人所取得的技术进展，以及它在生物学和医学中的应用。
Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial resolution. We recently discovered that preserved biological specimens can be physically expanded in an isotropic fashion through a chemical process. Expansion microscopy (ExM) allows nanoscale imaging of biological specimens with conventional microscopes, decrowds biomolecules in support of signal amplification and multiplexed readout chemistries, and makes specimens transparent. We review the principles of how ExM works, advances in the technology made by our group and others, and its applications throughout biology and medicine.