Bacterial flora in the gut of Helix aspersa (Gastropoda Pulmonata):: evidence for a permanent population with a dominant homolactic intestinal bacterium, Enterococcus casseliflavus
Bacterial flora in the gut of Helix aspersa (Gastropoda Pulmonata):: evidence for a permanent population with a dominant homolactic intestinal bacterium, Enterococcus casseliflavus
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DOI：
10.1139/cjm-44-1-20
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发表时间：
1998-01-01
影响因子：
2.8
通讯作者：
Ollivier, B
中科院分区：
文献类型：
--
作者：
Charrier, M;Combet-Blanc, Y;Ollivier, B
我们以前证明了存在的细菌降解羧甲基纤维素的肠道中的植食性蜗牛Hellaspersa，并发现肠杆菌科占主导地位的肠道中的蜗牛解剖在有氧条件下。在这里，我们调查了几种营养治疗对蜗牛的微生物区系的影响。食品灭菌导致蜗牛生长增加和作物中纤维素酶活性降低，表明微生物外源纤维素酶的有害作用。这项研究的第二个目的是寻找厌氧菌。没有严格的厌氧纤维素分解，同型产乙酸，或产甲烷细菌富集从肠道。然而，在厌氧条件下生长的能动革兰氏阳性同型乳酸球杆菌在蜗牛的肠道中占主导地位（1.57 x 10（9）+/- 0.10 x 10（9）个细胞/g（-1）肠道）。经鉴定为粪黄肠球菌。它存在于H. aspersa是讨论关于蜗牛的消化过程和存在的粪便粘液带。一种可能的螺菌协同作用的建议。
We previously demonstrated the existence of bacteria degrading carboxy-methyl-cellulose in the gut of the phytophagous snail Helix aspersa and found Enterobacteriaceae predominating in the intestine of snails dissected in aerobic conditions. Here, we investigated the effects of several nutritional treatments on the snail's microflora. Food sterilization led to increased snail growth and reduced cellulase activity in the crop, suggesting a noxious effect of microbial exogenous cellulases. A second aim of this study was to look for anaerobic bacteria. No strict anaerobic cellulolytic, homoacetogenic, or methanogenic bacteria were enriched from the gut. However, a motile Gram-positive homolactic coccobacillus, grown in anaerobic conditions, dominated in the snail's intestine (1.57 x 10(9) +/- 0.10 x 10(9) cells.g(-1) intestine). It was identified as Enterococcus casseliflavus. Its occurrence in the intestine of H. aspersa is discussed with regard to the snail's digestive processes and the presence of a fecal mucous ribbon. A possible snail-bacterium synergistic action is suggested.