The behavior of fibroblasts from the developing avian cornea. Morphology and movement in situ and in vitro.
The behavior of fibroblasts from the developing avian cornea. Morphology and movement in situ and in vitro.
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DOI：
10.1083/jcb.67.2.400
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发表时间：
1975-11
影响因子：
7.8
通讯作者：
Hay, E D
中科院分区：
文献类型：
--
作者：
Bard, J B;Hay, E D
早期雏鸡角膜由衬有前上皮和后内皮的无细胞胶原基质组成。在发育第 27-28 阶段（5 1/2 天），基质膨胀，使角膜厚度为 75-120 微米。与此同时，源自神经嵴的成纤维细胞开始侵入该间质。使用诺马尔斯基光学显微镜，我们将离体角膜中移动细胞的行为与人工胶原蛋白晶格和玻璃上相同细胞的迁移活动进行了比较。在原位，成纤维细胞具有圆柱体，从圆柱体延伸出一些粗的伪足和/或更细的丝状伪足。运动伴随着这些细胞质过程的活动。在玻璃上表征这些细胞的扁平褶皱片状足足在原位没有看到，但细胞运动的一般机制似乎与体外观察到的相同：细胞体（现在呈梨形）粗收缩或反冲到向前的细胞突中，或者细胞质更微妙地“流动”到向前的细胞突中，而不会立即损失尾随的细胞突。我们在原位和三维胶原蛋白晶格中拍摄了细胞之间的碰撞。这些成纤维细胞在成对碰撞中表现出经典的运动接触抑制（CIM），尽管它们缺乏褶皱边界。在玻璃上，这些细胞是多层的，这表明，虽然 CIM 影响细胞运动，但成纤维细胞可以相互利用作为基质。有丝分裂后细胞显示 CIM 相互远离。有趣的是，原位分裂细胞不会表现出表面起泡，但会在末期延长丝状伪足。讨论中强调了 CIM 在控制体内和体外细胞运动中的作用。
The early chick cornea is composed of an acellular collagenous stroma lined with an anterior epithelium and a posterior endothelium. At stage 27-28 of development (5 1/2 days), this stroma swells so that the cornea is 75-120 mum thick. At the same time, fibroblasts that originate from the neural crest begin to invade this stroma. Using Nomarski light microscopy, we have compared the behavior of moving cells in isolated corneas with the migratory activities of the same cells in artificial collagen lattices and on glass. In situ, fibroblasts have cyclindrical bodies from which extend several thick pseudopodia and/or finer filopodia. Movement is accompanied by activity in these cytoplasmic processes. The flat ruffling lamelli-podia that characterize these cells on glass are not seen in situ, but the general mechanism of cell movement seems to be the same as that observed in vitro: either gross contraction or recoil of the cell body (now pear shaped) into the forward cell process, or more subtle "flowing" of cytoplasm into the forward cell process without immediate loss of the trailing cell process. We filmed collisions between cells in situ and in three-dimensional collagen lattices. These fibroblasts show, in their pair-wise collisions, the classical contact inhibition of movement (CIM) exhibited in vitro even though they lack ruffled borders. On glass these cells multi-layer, showing that, while CIM affects cell movement, fibroblasts can use one another as a substratum. Postmitotic cells show CIM in moving away from each other. Interestingly, dividing cells in situ do not exhibit surface blebbing, but do extend filopodia at telophase. The role of CIM in controlling cell movement in vivo and in vitro is stressed in the discussion.