WHITEFLY TRANSMISSION AND EFFICIENT SSDNA ACCUMULATION OF BEAN GOLDEN MOSAIC GEMINIVIRUS REQUIRE FUNCTIONAL COAT PROTEIN
WHITEFLY TRANSMISSION AND EFFICIENT SSDNA ACCUMULATION OF BEAN GOLDEN MOSAIC GEMINIVIRUS REQUIRE FUNCTIONAL COAT PROTEIN
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DOI：
10.1006/viro.1994.1533
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发表时间：
1994-10-01
期刊：
影响因子：
3.7
通讯作者：
MAXWELL, DP
中科院分区：
文献类型：
--
作者：
AZZAM, O;FRAZER, J;MAXWELL, DP
豆金花叶双生病毒 (BGMV) 具有由两个环状 ssDNA 成分（DNA-A 和 DNA-B）组成的二分基因组，由烟粉虱传播。 DNA-A 编码病毒复制蛋白和外壳蛋白。为了确定 BGMV 外壳蛋白在全身感染和烟粉虱传播中的作用，在 BGMV 外壳蛋白基因中引入了两个缺失和一个限制性片段倒置。当使用放电粒子加速“粒子枪”将三种外壳蛋白突变体与 DNA-B 共接种到菜豆上时，所有三种外壳蛋白突变体均产生系统性感染。然而，它们不具有汁液传播性，并且在突变体感染的植物中未检测到外壳蛋白。此外，没有一个突变体是由粉虱传播的。对于所有三种突变体，与野生型 DNA 相比，DNA-A 和 DNA-B 的 ssDNA 积累分别减少了 25 至 50 倍和 3 至 10 倍。没有检测到对 dsDNA-A 积累的影响，并且 dsDNA-B 积累增加了 2 至 5 倍。当接种的外壳蛋白突变体在公共区域线性化时，突变的 DNA-A 和 DNA-B 形式之间的重组被鉴定，(C) 1994 年学术出版社，Inc.
Bean golden mosaic geminivirus (BGMV) has a bipartite genome composed of two circular ssDNA components (DNA-A and DNA-B) and is transmitted by the whitefly, Bemisia tabaci. DNA-A encodes the viral replication proteins and the coat protein. To determine the role of BGMV coat protein in systemic infection and whitefly transmission, two deletions and a restriction fragment inversion were introduced into the BGMV coat protein gene. All three coat protein mutants produced systemic infections when coinoculated with DNA-B onto Phaseolus vulgaris using electric discharge particle acceleration ''particle gun.'' However, they were not sap transmissible and coat protein was not detected in mutant-infected plants. In addition, none of the mutants were transmitted by whiteflies. With all three mutants, ssDNA accumulation of DNA-A and DNA-B was reduced 25- to 50-fold and 3- to 10-fold, respectively, as compared to that of wild-type DNA. No effect on dsDNA-A accumulation was detected and there was 2- to 5-fold increase in dsDNA-B accumulation. Recombinants between the mutated DNA-A and DNA-B forms were identified when the inoculated coat protein mutant was linearized in the common region, (C) 1994 Academic Press, Inc.