The Potential of Isolation Source to Predict Colonization in Avian Hosts: A Case Study in Campylobacter jejuni Strains From Three Bird Species.
The Potential of Isolation Source to Predict Colonization in Avian Hosts: A Case Study in Campylobacter jejuni Strains From Three Bird Species.
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DOI：
10.3389/fmicb.2018.00591
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发表时间：
2018
影响因子：
5.2
通讯作者：
Ellström P
中科院分区：
文献类型：
--
作者：
Atterby C;Mourkas E;Méric G;Pascoe B;Wang H;Waldenström J;Sheppard SK;Olsen B;Järhult JD;Ellström P
空肠弯曲杆菌是全球细菌性胃肠炎的主要原因，主要通过食用受污染的家禽感染人类。C.空肠在野生鸟类的肠道中是常见的，并且显示出与特定鸟类物种的明显的菌株特异性关联。这与农场动物形成对比，农场动物中几种基因型共存。目前还不清楚限制这种特殊菌株在宿主种属之间传播的障碍是否与环境因素有关，如宿主种属之间的接触、环境中的细菌存活等，或者更确切地说，是为了使特异性适应特定宿主的肠道环境。我们比较了两种宿主特异性C.来自歌鸫（ST-1304复合体）和绿头鸭（ST-995）的空肠，以及来自鸡（ST-21复合体）的多面手菌株在野生宿主绿头鸭（Anas platyrhynchos）中。在18天的感染实验中，歌鸫株表现出只有弱的殖民化，并清除所有鸟类10天后，而野鸭和鸡株保持稳定。当鸡株在与野鸭株共同感染同一只鸡之前4天给药时，后者很快就超过了鸡株。相比之下，当野鸭菌株与鸡菌株共感染前4天给予时，野鸭菌株仍然存在，鸡菌株的扩增被延迟。我们的结果表明C.空肠定植野鸭，可能与主机的起源。这种差异可以解释观察到的C。野鸟的空肠
Campylobacter jejuni is the primary cause of bacterial gastroenteritis worldwide, infecting humans mostly through consumption of contaminated poultry. C. jejuni is common in the gut of wild birds, and shows distinct strain-specific association to particular bird species. This contrasts with farm animals, in which several genotypes co-exist. It is unclear if the barriers restricting transmission between host species of such specialist strains are related to environmental factors such as contact between host species, bacterial survival in the environment, etc., or rather to strain specific adaptation to the intestinal environment of specific hosts. We compared colonization dynamics in vivo between two host-specific C. jejuni from a song thrush (ST-1304 complex) and a mallard (ST-995), and a generalist strain from chicken (ST-21 complex) in a wild host, the mallard (Anas platyrhynchos). In 18-days infection experiments, the song thrush strain showed only weak colonization and was cleared from all birds after 10 days, whereas both mallard and chicken strains remained stable. When the chicken strain was given 4 days prior to co-infection of the same birds with a mallard strain, it was rapidly outcompeted by the latter. In contrast, when the mallard strain was given 4 days prior to co-infection with the chicken strain, the mallard strain remained and expansion of the chicken strain was delayed. Our results suggest strain-specific differences in the ability of C. jejuni to colonize mallards, likely associated with host origin. This difference might explain observed host association patterns in C. jejuni from wild birds.