Induction of apoptosis in cancer cells by bilberry (Vaccinium myrtillus) and the anthocyanins
Induction of apoptosis in cancer cells by bilberry (Vaccinium myrtillus) and the anthocyanins
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DOI：
10.1021/jf025781x
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发表时间：
2003-01-01
影响因子：
6.1
通讯作者：
Kobori, M
中科院分区：
文献类型：
--
作者：
Katsube, N;Iwashita, K;Kobori, M
在 10 种可食用浆果的乙醇提取物中，越橘提取物被发现对体外抑制 HL60 人类白血病细胞和 HCT116 人类结肠癌细胞的生长最有效。越橘提取物诱导 HL60 细胞凋亡细胞体和核小体 DNA 断裂。 HCT116中越橘提取物诱导的细胞凋亡比例远低于HL60细胞，且前者不诱导DNA断裂。在测试的提取物中，越橘提取物含有最多量的酚类化合物，包括花青素，并且显示出最大的 1,1-二苯基-2-三硝基苯肼 (DPPH) 自由基清除活性。纯飞燕草素和锦葵素，与从越橘提取物中分离出的糖苷一样，可诱导 HL60 细胞凋亡。这些结果表明，越橘提取物和花青素（以飞燕草素或锦葵素为苷元）通过诱导细胞凋亡来抑制 HL60 细胞的生长。只有纯飞燕草素和从越橘提取物中分离的糖苷，而不是锦葵素和糖苷，可以抑制 HCT116 细胞的生长。
Among ethanol extracts of 10 edible berries, bilberry extract was found to be the most effective at inhibiting the growth of HL60 human leukemia cells and HCT116 human colon carcinoma cells in vitro. Bilberry extract induced apoptotic cell bodies and nucleosomal DNA fragmentation in HL60 cells. The proportion of apoptotic cells induced by bilberry extract in HCT116 was much lower than that in HL60 cells, and DNA fragmentation was not induced in the former. Of the extracts tested, that from bilberry contained the largest amounts of phenolic compounds, including anthocyanins, and showed the greatest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Pure delphinidin and malvidin, like the glycosides isolated from the bilberry extract, induced apoptosis in HL60 cells. These results indicate that the bilberry extract and the anthocyanins, bearing delphinidin or malvidin as the aglycon, inhibit the growth of HL60 cells through the induction of apoptosis. Only pure delphinidin and the glycoside isolated from the bilberry extract, but not malvidin and the glycoside, inhibited the growth of HCT116 cells.