MECHANISMS OF CELLULAR ADHESION .3. PREPARATION AND PRELIMINARY CHARACTERIZATION OF ADHESIONS
MECHANISMS OF CELLULAR ADHESION .3. PREPARATION AND PRELIMINARY CHARACTERIZATION OF ADHESIONS
复制标题
DOI：
10.1016/0014-4827(78)90136-2
复制
发表时间：
1978-01-01
影响因子：
3.7
通讯作者：
REES, DA
中科院分区：
文献类型：
--
作者：
BADLEY, RA;LLOYD, CW;REES, DA
在玻璃或塑料基质上生长的成纤维细胞下发现的特化粘连从细胞体中分离出来，用干涉反射和免疫荧光显微镜检查。对鉴定细胞的研究表明，残留的物质确实与活细胞的局灶粘连相对应。使用特异性抗血清可以在黏附中鉴定出以下成分：肌动球蛋白系统的主要蛋白质；张力丝系统的主要组成部分；这些位点对蓖麻毒素有特异性，但对豆毒蛋白A或16C（大鼠真皮）成纤维细胞表面决定因子和与人血清冷不溶性球蛋白相关的成分则不那么有特异性。未检出微管蛋白和血清白蛋白。这些成分在将信息从细胞外部传递到细胞内部的过程中所起的作用与细胞对底物的反应有关。
The specialized adhesions found under fibroblasts growing on a glass or plastic substrate were detached from the cell bodies and examined by interference reflection and immunofluorescence microscopy. Studies with identified cells showed that the material remaining does correspond to the focal adhesions of the living cells. Use of specific antisera has lead to the identification of the following components in the adhesions: major proteins of the actomyosin system; the major component of the tonofilament system; sites specific for ricin but much less so for concanavalin A or the 16C [rat dermal] fibroblast surface determinants and a component related to the cold insoluble globulin of human serum. Tubulin and serum albumin were not detected. The part these components play in transmitting information from the outside to the inside of the cell is discussed in relation to the cellular response to a substrate.