Direct release of parathyroid hormone fragments from functioning bovine parathyroid glands in vitro.
Direct release of parathyroid hormone fragments from functioning bovine parathyroid glands in vitro.
复制标题
体外功能牛甲状旁腺直接释放甲状旁腺激素片段。
DOI：
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发表时间：
1978
影响因子：
15.9
通讯作者：
L. Sherwood
中科院分区：
文献类型：
--
作者：
D. Hanley;K. Takatsuki;J. Sultan;A. Schneider;L. Sherwood
To determine the origin of circulating parathyroid hormone fragments, hormonal peptides released from bovine parathyroid tissue in a physiologically responsive in vitro "perifusion" system were analyzed by gel exclusion chromatography and region-specific radioimmunoassays. When exposed to low Ca++, the tissue released large quantities of intact hormone (parathyroid hormone 1--84) as well as amino- and carboxyl-terminal fragments. Fragments of the hormone were also released when the tissue was exposed to high Ca++, but the carboxyl fragments comprised a much greater proportion of the hormonal peptides released. Control experiments indicated that fragmentation of the hormone occurred within the gland and not after it was secreted. These experiments provide direct evidence, therefore, that release of fragments from the parathyroid gland may contribute to the immunologic heterogeneity of the hormone in the circulation.
