In vivo description of dendritic cells in human renal cell carcinoma.
In vivo description of dendritic cells in human renal cell carcinoma.
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DOI：
10.1016/s0022-5347(05)68628-4
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发表时间：
1999-08
期刊：
影响因子：
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通讯作者：
T. Schwaab;A. Schned;J. Heaney;B. Cole;J. Atzpodien;F. Wittke;M. Ernstoff
中科院分区：
文献类型：
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作者：
T. Schwaab;A. Schned;J. Heaney;B. Cole;J. Atzpodien;F. Wittke;M. Ernstoff
目的树突状细胞（Dendritic cells，DCs）是一种高效的抗原提呈细胞，在启动初次免疫应答中起重要作用。它们是T细胞活化的最有效的刺激物，因此预期在抗肿瘤免疫应答中具有重要意义。虽然DC的表型和功能已在体外条件下进行了描述，其在体内的特点是不够详细。人肾细胞癌（RCC）是研究肿瘤浸润性树突状细胞（TiDCs）的极好模型，因为其罕见的临床自发消退和大量的肿瘤浸润性淋巴细胞（TiLs）的相关性，表明强烈的免疫应答。（CD 86 [B7.2]、CD 80 [B7.1]、CD 40、CD 54、CD 1a和HLA-DR）。采用免疫组化双染法和光镜技术对17例原发性RCC进行检测，分别代表4种不同的组织学类型。CD 40的表达与CD 83 + TiDC上的CD 1a的表达相关。CD 54（ICAM-1）的表达与CD 86（B7.2）的表达降低，以及在CD 3+和CD 8 +TILs.ConclusionsThese数据表明从头脂质或糖为基础的免疫原性抗原提呈TiDCs的减少。此外，基于CD 86（B7.2）的共表达减少和相关的CD 8 + TiL的减少，数据支持CD 54 + TiDC的抗原呈递能力受损。
PurposeDendritic cells (DCs) are efficient and effective antigen-presenting cells that play a major role in initiating the primary immune response. They are the most potent stimulators of T-cell activation and would thus be expected to be of great importance in the antitumoral immune response. Although DC phenotype and function have been described under in vitro conditions, their in vivo characteristics are less well detailed. Human renal cell carcinoma (RCC) is an excellent model to explore tumor infiltrating dendritic cells (TiDCs) because of rare clinical spontaneous regressions and the association of high numbers of tumor infiltrating lymphocytes (TiLs), suggesting a strong immune response.Materials and MethodsWe determined the in situ phenotype of mature CD83+TiDCs using monoclonal antibodies to known activation molecules (CD86 [B7.2], CD80 [B7.1], CD40, CD54, CD1a and HLA-DR). Seventeen primary RCCs, representing four distinct histologies, were evaluated using double-staining immunohistochemical techniques and light microscopy.ResultsCD83+TiDCs were found in all tumors. Expression of CD40 correlated with expression of CD1a on CD83+TiDCs. Expression of CD54 (ICAM-1) correlated with a lower expression of CD86 (B7.2) as well as a decrease in CD3+and CD8+TiLs.ConclusionsThese data suggest a de novo lipid or sugar-based immunogenic antigen presentation by TiDCs. Also, the data support an impaired antigen-presenting capability for CD54+TiDCs based on the decreased coexpression of CD86 (B7.2) and the decrease of associated CD8+TiLs.