YACS AND THE C-ELEGANS GENOME
YACS AND THE C-ELEGANS GENOME
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DOI：
10.1002/bies.950130809
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发表时间：
1991-08-01
期刊：
影响因子：
4
通讯作者：
WATERSTON, R
中科院分区：
文献类型：
--
作者：
COULSON, A;KOZONO, Y;WATERSTON, R
在过去的十年中，很明显，充分了解复杂生物体的发育和功能是我们力所能及的。人们普遍认为，这项工作必须包括阐明一些模式生物的遗传蓝图——基因组序列。作为确定这些序列的前奏，基于克隆的许多多细胞动物和植物基因组物理图谱正在构建中。酵母人工染色体（YAC）载体由于其相对无偏倚的克隆能力和携带大插入物的能力，在这些图谱的构建中起着核心作用。将YACs应用于秀丽隐杆线虫基因组的物理图谱，使宇宙克隆“岛”能够以一种有效的方式连接在一起。长期的连续性改善了遗传图谱和物理图谱之间的联系，大大增加了它的效用。由于基因组可以由相对较少数量的YACs代表，因此有可能为整个社区提供基因组有序的YACs的复制过滤器。
During the past decade, it has become apparent that it is within our grasp to understand fully the development and functioning of complex organisms. It is widely accepted that this undertaking must include the elucidation of the genetic blueprint - the genome sequence - of a number of model organisms. As a prelude to the determination of these sequences, clone-based physical maps of the genomes of a number of multicellular animals and plants are being constructed. Yeast artificial chromosome (YAC) vectors, by virtue of their relatively unbiased cloning capabilities and capacity to carry large inserts, have come to play a central role in the construction of these maps. The application of YACs to the physical map of the Caenorhabditis elegans genome has enabled cosmid clone 'islands' to be linked together in an efficient manner. The long-range continuity has improved the linkage between the genetic and physical maps, greatly increasing its utility. Since the genome can be represented by a relatively small number of YACs, it has been possible to make replica filters of genomically ordered YACs available to the community at large.