Tissue refractive index as marker of disease
Tissue refractive index as marker of disease
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DOI：
10.1117/1.3656732
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发表时间：
2011-11-01
影响因子：
3.5
通讯作者：
Popescu, Gabriel
中科院分区：
文献类型：
--
作者：
Wang, Zhuo;Tangella, Krishnarao;Popescu, Gabriel
组织病理学的黄金标准依赖于染色组织活检的手动研究。非常需要一种用于原位组织标本检查的灵敏且定量的方法，因为它可以实现早期疾病诊断和自动筛查。在这里，我们证明整个未染色活检的定量相位成像有可能满足这一要求。我们的数据表明，组织病理学载玻片的折射率分布包含有关组织分子尺度组织的信息，可揭示前列腺肿瘤和乳腺钙化。这些光学图报告了组织和细胞的微妙的纳米级形态特性，这些特性无法通过普通染色剂（包括苏木精和曙红）恢复。我们发现癌症进展显着改变了组织组织，前列腺肿瘤与正常区域的折射率方差一致较高就表明了这一点。此外，利用定量相位信息，我们获得了整个活检的空间分辨散射平均自由程和各向异性因子 g，并证明了它们与肿瘤存在的直接相关性。本质上，我们的结果表明，组织折射率报告了纳米级组织结构，原则上可以用作癌症诊断的内在标记。 (C) 2011 年光电仪器工程师协会 (SPIE)。 [DOI：10.1117/1.3656732]
The gold standard in histopathology relies on manual investigation of stained tissue biopsies. A sensitive and quantitative method for in situ tissue specimen inspection is highly desirable, as it would allow early disease diagnosis and automatic screening. Here we demonstrate that quantitative phase imaging of entire unstained biopsies has the potential to fulfill this requirement. Our data indicates that the refractive index distribution of histopathology slides, which contains information about the molecular scale organization of tissue, reveals prostate tumors and breast calcifications. These optical maps report on subtle, nanoscale morphological properties of tissues and cells that cannot be recovered by common stains, including hematoxylin and eosin. We found that cancer progression significantly alters the tissue organization, as exhibited by consistently higher refractive index variance in prostate tumors versus normal regions. Furthermore, using the quantitative phase information, we obtained the spatially resolved scattering mean free path and anisotropy factor g for entire biopsies and demonstrated their direct correlation with tumor presence. In essence, our results show that the tissue refractive index reports on the nanoscale tissue architecture and, in principle, can be used as an intrinsic marker for cancer diagnosis. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3656732]