Selective β2-adrenergic Antagonist Butoxamine Reduces Orthodontic Tooth Movement
Selective β2-adrenergic Antagonist Butoxamine Reduces Orthodontic Tooth Movement
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DOI：
10.1177/0022034514536730
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发表时间：
2014-08-01
影响因子：
7.6
通讯作者：
Goto, S.
中科院分区：
文献类型：
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作者：
Sato, T.;Miyazawa, K.;Goto, S.
最近，交感神经系统参与骨代谢引起了人们的关注。β 2-肾上腺素能受体（β 2-AR）存在于成骨细胞和骨细胞上。我们以前证明，低剂量的β-AR阻滞剂通过β 2-AR阻滞改善交感神经系统过度活跃的骨质疏松症，而高剂量的β-AR阻滞剂可能对成骨细胞活性有一定的抑制作用。本研究观察了β_2-AR特异性拮抗剂butoxamine（BUT）对自发性高血压大鼠（SHR）牙齿移动的影响。我们口服BUT（1 mg/kg），并将闭合螺旋弹簧插入左上第一磨牙。处死后，我们计算牙齿移动量，并分析小梁微结构和组织形态计量学。SHR对照组的距离大于Wistar-Kyoto大鼠组，但BUT处理组与Wistar-Kyoto大鼠对照组相比无显著差异。对每单位组织体积的骨体积、骨小梁数量和每单位骨表面的破骨细胞表面在牙槽骨中的分析表明，SHR中骨吸收的增加导致了明显的骨丢失。此外，BUT治疗导致牙槽骨丢失的恢复。此外，TH-免疫反应神经在牙周膜增加牙齿移动，和BUT管理减少TH-免疫反应神经。这些结果表明，BUT通过阻断β 2-AR防止牙槽骨丢失和正畸牙齿移动。
Recently, involvement of the sympathetic nervous system in bone metabolism has attracted attention. beta 2-Adrenergic receptor (beta 2-AR) is presented on osteoblastic and osteoclastic cells. We previously demonstrated that beta-AR blockers at low dose improve osteoporosis with hyperactivity of the sympathetic nervous system via beta 2-AR blocking, while they may have a somewhat inhibitory effect on osteoblastic activity at high doses. In this study, the effects of butoxamine (BUT), a specific beta 2-AR antagonist, on tooth movement were examined in spontaneously hypertensive rats (SHR) showing osteoporosis with hyperactivity of the sympathetic nervous system. We administered BUT (1 mg/kg) orally, and closed-coil springs were inserted into the upper-left first molar. After sacrifice, we calculated the amount of tooth movement and analyzed the trabecular microarchitecture and histomorphometry. The distance in the SHR control was greater than that in the Wistar-Kyoto rat group, but no significant difference was found in the SHR treated with BUT compared with the Wistar-Kyoto rat control. Analysis of bone volume per tissue volume, trabecular number, and osteoclast surface per bone surface in the alveolar bone showed clear bone loss by an increase of bone resorption in SHR. In addition, BUT treatment resulted in a recovery of alveolar bone loss. Furthermore, TH-immunoreactive nerves in the periodontal ligament were increased by tooth movement, and BUT administration decreased TH-immunoreactive nerves. These results suggest that BUT prevents alveolar bone loss and orthodontic tooth movement via beta 2-AR blocking.