Identification of microRNA-target genes in mice hippocampus at 1 week after pilocarpine-induced status epilepticus
Identification of microRNA-target genes in mice hippocampus at 1 week after pilocarpine-induced status epilepticus
复制标题
毛果芸香碱诱导癫痫持续状态 1 周后小鼠海马 microRNA 靶基因的鉴定
DOI：
10.1016/j.bbrc.2020.06.125
复制
发表时间：
2020-10-20
影响因子：
3.1
通讯作者：
Liu，Jian-Xin
中科院分区：
文献类型：
--
作者：
Xiao，Xin-Li;Wu，Xiao-Lin;Liu，Jian-Xin
MicroRNA（miRNA）被认为通过控制疾病不同阶段的基因表达，在颞叶癫痫（TLE）的病因和治疗中发挥着至关重要的作用。为了研究 miRNA 在癫痫持续状态后潜伏期的作用，我们首先使用 Exiqon miRCURY LNA™ miRNA 阵列比较了毛果芸香碱诱导癫痫持续状态 (SE) 后 1 周时小鼠海马组织中的 microRNA 表达谱与对照海马组织中的 microRNA 表达谱。然后，使用 TargetScan 7.1 和 miRDB V5 预测改变的 miRNA 的靶基因，并通过与同一时间点样本中的另一个独立 mRNA 表达谱数据集交叉来进一步选择。我们在 SE 小鼠中发现了 14 个常见基因作为下调 miRNA 目标（上调 mRNA）和 4 个常见基因作为上调 miRNA 目标（下调 mRNA）。 miR-669m-3p-TRHR（促甲状腺素释放激素受体）、miR-669m-3p-B3galt2（β-1,3-半乳糖基转移酶2）、miR-105-PDPN（Podoplanin）和miR-883b-3p-CLEC-2（C-type-lectin-like-2）被发现是调节钙信号通路的潜在分子机制。分别在毛果芸香碱诱导 SE 后 1 周，小鼠海马的糖基化途径和趋化因子介导的炎症过程。我们的结果为小鼠海马中由 miRNAS 靶基因介导的细胞事件提供了潜在的新见解，这些细胞事件塑造了 SE 诱发的癫痫发生。
MicroRNAs (miRNA) are believed to play a crucial role in the cause and treatment of temporal lobe epilepsy (TLE) by controlling gene expression in different stages of the disease. To investigate role of miRNA in the latent stage following status epilepticus, we first compared microRNA expression profiles in mice hippocampus at 1 week after pilocarpine-induced status epilepticus (SE) vs. controls in hippocampal tissues using Exiqon miRCURY LNA™ miRNAs Array. Then, the target genes of altered miRNAs were predicted using both TargetScan 7.1 and miRDB V5, and were further selected by intersecting with another independent mRNA expression profile dataset from the samples at the same time point. We found out 14 common genes as down miRNA target (up-mRNA) and 4 common genes as up miRNA target (down mRNA) in SE mice. miR-669m-3p-TRHR (thyrotropin releasing hormone receptor), miR-669m-3p-B3galt2 (β-1,3-Galactosyltransferase 2), miR-105-PDPN (Podoplanin) and miR-883b-3p-CLEC-2 (C-type-lectin-like-2) were found to be potential molecular mechanisms to modulate the calcium signaling pathway, glycosylation pathways and chemokine mediated inflammatory processes in mice hippocampus at 1 week after pilocarpine-induced SE, respectively. Our results offered potential novel insights into the cellular events in the mice hippocampus mediated by miRNASs-target genes that shape SE-evoked epileptogenesis.