1,25-Dihydroxyvitamin D3 selectively translocates PKCα to nuclei in ROS 17/2.8 cells
1,25-Dihydroxyvitamin D3 selectively translocates PKCα to nuclei in ROS 17/2.8 cells
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DOI：
10.1016/s0303-7207(01)00670-0
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发表时间：
2002-02-25
影响因子：
4.1
通讯作者：
Mellon, WS
中科院分区：
文献类型：
--
作者：
Rivera-Bermúdez, MA;Bertics, PJ;Mellon, WS
We have investigated protein kinase C (PKC) regulation by 1,25-(OH)(2)D-3 in the rat osteosarcoma cell line ROS 17/2.8 since previous reports have implicated PKC in the 1,25-(OH)(2)D-3-mediated regulation of osteocalcin gene expression (J. Biol. Chem. 267 (1992) 12562; Endocrinology 136 (1995) 5685). Here we report that 1,25-(OH)(2)D-3 increased PKCalpha, but not PKCbetaI, epsilon or xi, levels in the nuclear fraction in a time-dependent manner. Unlike PMA, 1,25-(OH)(2)D-3 did not alter the association of any of the expressed PKC isoenzymes with the plasma membrane. Treatment with 20 nM 1,25-(OH)(2)D-3 for 15 min, 30 min, 1 h and 24 h increased PKCalpha levels in the nuclear fraction by 2.3- to 2.6-fold. Nuclear PKCalpha expression was also increased with doses of 1,25-(OH)(2)D-3 as low as a 0.05 nM. 1,25-(OH)(2)D-3-mediated stabilization of osteocalcin mRNA (Arch. Biochem. Biophys. 332 (1996) 142) was inhibited with bisindolylmaleimide treatment, suggesting that PKCalpha may be involved in the 1,25-(OH)(2)D-3-mediated regulation of osteocalcin gene expression. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
