High-performance liquid chromatographic analysis of bioactive triterpenes in Perilla frutescens
High-performance liquid chromatographic analysis of bioactive triterpenes in Perilla frutescens
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DOI：
10.1016/s0731-7085(03)00160-2
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发表时间：
2003-08-21
影响因子：
3.4
通讯作者：
Tan, RX
中科院分区：
文献类型：
--
作者：
Chen, JH;Xia, ZH;Tan, RX
紫苏（L.）布瑞特。（Lamiaceae）是一种著名的中药，被用于治疗各种疾病。为了评价金银花药材的质量，建立了一种简便、快速、准确的高效液相色谱法（HPLC）测定金银花药材中3种生物活性三萜酸：拷问酸（TA）、齐墩果酸（OA）和熊果酸（UA）。色谱柱为Spherisob十八烷基硅基二氧化硅（ODS）柱，流动相为乙腈和水溶液H3PO4，检测波长为206 nm。方法准确度高，相对标准偏差在0.6-1.5%（日内）和0.7-2.6%（间）之间。测定了生长在中国8个不同产地的三叶草叶片中这3种植物化学物质的含量，以确定该方法的有效性。(C) 2003 Elsevier Science B.V.版权所有
Perilla frutescens (L.) Britt. (Lamiaceae), a famous traditional Chinese medicine, has been used for the treatment of various diseases. To evaluate the quality of P. frutescens, a simple, rapid and accurate high-performance liquid chromatography (HPLC) method was developed for the assessment of three bioactive triterpene acids: tormentic acid (TA), oleanolic acid (OA) and ursolic acid (UA). The HPLC system used an Spherisob octadecylsilyl silica (ODS) column with acetonitrile and aqueous H3PO4 as the mobile phase and detection at 206 nm. The method was precise with relative standard deviations for these three constituents that ranged between 0.6-1.5% (intraday) and 0.7-2.6% (interday). The content of these three phytochemicals in the leaves of P. frutescens growing at eight different locations of China was determined to establish the effectiveness of the method. (C) 2003 Elsevier Science B.V. All rights reserved.