Calcium channels in isolated cells and strips of longitudinal muscle of rat myometrium.
Calcium channels in isolated cells and strips of longitudinal muscle of rat myometrium.
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大鼠子宫肌层分离细胞和纵向肌条中的钙通道。
DOI：
10.1139/y87-307
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发表时间：
1987
影响因子：
2.1
通讯作者：
E. Daniel
中科院分区：
文献类型：
--
作者：
M. Kyozuka;J. Crankshaw;E. Daniel
研究了在K+浓度升高、部分通道激动剂Bay K 8644和尼群地平作用下，雌激素主导的大鼠子宫肌层纵肌条带和单个肌细胞中Ca2+通道的特性。在 0.5 mM Ca2+ 的隔离条中，Bay K 8644 (pD2 = 7.8-8.0) 降低了响应 K+ 浓度升高的阈值并增强了收缩，包括单独对 K+ 升高的最大响应。浓度高达 10(-6) M 的 Bay K 8644 单独使用不会在 0.5 mM Ca2+ 溶液中引发收缩。在较高浓度 (10(-5) M) 下，Bay K 8644 可作为 K+ 升高引起的收缩的拮抗剂。在分离的细胞中，10(-7) M Bay K 8644 增强了 K+ 升高的缩短，并降低了所需的 K+ 浓度阈值。在正常 K+ 浓度下，10(-7) M Bay K 8644 也没有发生明显的收缩。与其在孤立条带中的效果相反，没有观察到最大缩短（至 60 mM K+）的显着增加，可能是因为没有机械负载的细胞仅被 K+ 最大限度地缩短。从这些研究中，我们得出结论，大鼠子宫肌层的孤立条带和细胞的 Ca2+ 通道与其他平滑肌的 Ca2+ 通道在与 K+、尼群地平和 Bay K 8644 升高的相互作用方面具有相似的行为和特性。
The properties of Ca2+ channels in strips and single muscle cells of longitudinal muscle of estrogen-dominated rat myometrium were studied under the effects of elevation of K+ concentration, the partial channel agonist Bay K 8644, and nitrendipine. In isolated strips in 0.5 mM Ca2+, Bay K 8644 (pD2 = 7.8-8.0) lowered the threshold for and enhanced the contractions in response to an elevation of K+ concentration, including the maximum response to K+ elevation alone. Bay K 8644 alone in concentrations up through 10(-6) M did not initiate contractions in 0.5 mM Ca2+ solutions. At higher concentrations (10(-5) M), Bay K 8644 behaved as an antagonist to contractions induced by elevation of K+. In isolated cells 10(-7) M Bay K 8644 enhanced the shortenings to elevated K+ and lowered the threshold K+ concentration required. Also no significant contraction occurred with 10(-7) M Bay K 8644 at normal K+ concentration. In contrast with its effect in isolated strips, no significant increase in maximum shortening (to 60 mM K+) was observed, possibly because cells without a mechanical load were maximally shortened by K+ alone. From these studies, we conclude that Ca2+ channels of isolated strips and cells of rat myometrium behave similarly and have similar properties to those of other smooth muscles in their interactions with elevation of K+, nitrendipine, and Bay K 8644.
尼群地平和 Bay k 8644 与离体犬心脏肌膜结合与其对犬心脏药理作用的相关性。
影响因子：
5
作者：
Vaghy,PL;Grupp,IL;Grupp,G;Balwierczak,JL;Williams,JS;Schwartz,A
通讯作者：
Schwartz,A