Expression of arginase I and inducible nitric oxide synthase in the peripheral blood and lymph nodes of HIV‑positive patients.
Expression of arginase I and inducible nitric oxide synthase in the peripheral blood and lymph nodes of HIV‑positive patients.
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HIV阳性患者外周血和淋巴结中精氨酸酶I和诱导型一氧化氮合酶的表达
DOI：
10.3892/mmr.2015.4601
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发表时间：
2016-01
影响因子：
3.4
通讯作者：
Zhao M
中科院分区：
文献类型：
--
作者：
Zhang N;Deng J;Wu F;Lu X;Huang L;Zhao M
精氨酸酶I（Arg I）和诱导型一氧化氮合酶（iNOS）通过其代谢产物在调节免疫功能中是重要的。以往的研究表明，在人类免疫缺陷病毒（HIV）感染患者的血清和外周血单个核细胞中，Arg I的表达增加，而iNOS的表达减少。淋巴结作为最重要的免疫器官和HIV复制场所之一，在HIV感染后是否也存在类似的变化尚待阐明。为了研究这一点，本研究收集了52例HIV感染者的淋巴结和血液标本，通过免疫组织化学和基于荧光的流式细胞术测量Arg I和iNOS的表达水平。与对照组相比，HIV感染者淋巴结中Arg I的表达水平显著升高，血液和淋巴结中Arg I+ CD4+ T细胞和CD8+ T细胞的比例显著升高，而相应区域中iNOS的表达水平则相反。淋巴结和血液中Arg I的表达水平与外周血CD4+ T细胞计数呈负相关，与病毒载量呈正相关。淋巴结和血液中iNOS的表达水平与外周血CD4+ T细胞计数呈正相关，与病毒载量呈负相关。这些结果表明，HIV感染患者外周T细胞和外周淋巴结中Arg I和iNOS表达水平的改变与这些患者的疾病进展相关。这些结果表明，通过调节和操纵HIV感染患者中Arg I和iNOS的表达水平来延缓疾病进展的治疗策略是潜在的。
Arginase I (Arg I) and inducible nitric oxide synthase (iNOS) are important in regulating immune functions through their metabolites. Previous studies have revealed that the expression of Arg I is increased and the expression of iNOS is reduced in the serum and peripheral blood mononuclear cells of human immunodeficiency virus (HIV)-infected patients. As one of the most important immune organs and HIV replication sites, whether similar changes are present in the lymph nodes following HIV infection remains to be elucidated. To investigate this, the present study collected lymph node and blood specimens from 52 HIV-infected patients to measure the expression levels of Arg I and iNOS by immunohistochemistry and fluoresence-based flow cytometry. Compared with control subjects without HIV infection, the patients with HIV had significantly higher expression levels of Arg I in the lymph nodes and higher frequencies of Arg I+ CD4+ T cells and CD8+ T cells in the blood and lymph nodes, and these results were contrary the those of iNOS in the corresponding compartments. The expression levels of Arg I in the lymph nodes and blood were negatively associated with peripheral CD4+ T cell count and positively associated with viral load. However, the expression levels of iNOS in the lymph nodes and blood were positively associated with peripheral CD4+ T cell count and negatively associated with viral load. These results showed that alterations in the expression levels of Arg I and iNOS in the peripheral T cells and peripheral nodes of HIV infected patients are associated with disease progression in these patients. These results indicate a potential to therapeutic strategy for delaying disease progression through regulating and manipulating the expression levels of Arg I and iNOS in patients infected with HIV.