Genome organization and phylogenetic distribution of a novel family of ancient murine endogenous proviruses with evidence for transposition-mediated proliferation.
Genome organization and phylogenetic distribution of a novel family of ancient murine endogenous proviruses with evidence for transposition-mediated proliferation.
复制标题
古代鼠内源性原病毒新家族的基因组组织和系统发育分布，以及转座介导增殖的证据。
DOI：
10.1023/a:1002075821782
复制
发表时间：
2000
影响因子：
2.4
通讯作者：
Luo,M
中科院分区：
文献类型：
--
作者：
Zimmerer,EJ;Carneal,J;Robertson,JB;Ozturk,M;Cardiff,S;Luo,M
这里描述了一个新的鼠内源性原病毒家族 (VL6.0)。完整的原病毒长度接近 6 kb，显示出 5" LTR、gag、pol、env 和 3" LTR 的基因组组织。引物结合位点 (PBS) 是 tRNAgly 的结合位点。该群体的大多数（如果不是全部）成员缺乏功能性开放阅读框架，并且存在显着的空白，这表明它是古老的。我们估计每个单倍体基因组的拷贝数为 30+。该组的成员已从 Mus musculus Domesticus，M. m. 中分离出来。卡斯特纽斯，M. m. hortulanus，M.卡罗利，和M。斯普雷图斯。这些序列在麝香属如此多样化的成员中出现，可能表明最初感染的日期早于现存麝香谱系的分化，距今约 250 万年前。对间隙（缺失/插入）模式的分析表明，这些序列可能通过逆转录酶介导的转座机制在Musgenome内增殖。迄今为止，还没有描述密切相关的鼠逆转录病毒。根据序列相似性，最接近的哺乳动物逆转录病毒来自小型猪（Sus scrofa）。
A new family of murine endogenous proviruses (VL6.0) is described here. The intact provirus is near 6 kb in length and shows a genomic organization of 5" LTR,gag,pol,env, and 3" LTR. The primer binding site (PBS) is that of a tRNAgly. The lack of functional open reading frames and occurrence of significant gaps in most, if not all, members of this group show it to be ancient. Our estimate of copy number per haploid genome is 30+. Members of this group have been isolated fromMus musculus domesticus, M. m. casteneus, M. m. hortulanus, M. caroli, andM. spretus. The occurrence of these sequences throughout such diverse members of the genusMusmay indicate that the date of the original infection predated the divergence of the extantMuslineages at around 2.5 million years ago. Analysis of gap (deletion/insertion) patterns indicates that these sequences may have proliferated within theMusgenome by a mechanism of reverse transcriptase-mediated transposition. As yet, there are no closely related murine retroviruses described. The closest mammalian retrovirus based on sequence similarity is from the miniature swine (Sus scrofa).