Replication Origins Why Do We Need So Many?
Replication Origins Why Do We Need So Many?
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DOI：
10.4161/cc.2.4.435
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发表时间：
2003-01-01
期刊：
影响因子：
4.3
通讯作者：
Bielinsky, Anja-Katrin
中科院分区：
文献类型：
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作者：
Bielinsky, Anja-Katrin
在细胞周期的G（1）期，复制起点准备发射，这一过程被称为起源许可。人们经常思考，如果不是所有的复制起点都得到许可并随后在S期被激活，细胞的命运会是什么。一个明显的预测是，S期只会延长。然而，事实证明，后果要复杂得多。由于过早进入S期或其他对起源许可产生负面影响的事件而导致的短G（1）期最终将损害细胞在进入有丝分裂之前完成DNA复制的能力。因此，当细胞在分裂后期试图修复未复制的DNA时，它的基因组变得不稳定。因此，真核细胞染色体中活跃复制起点的密度决定了有丝分裂过程中S期的动态和染色体的稳定性。
During the G(1) phase of the cell cycle, replication origins are prepared to fire, a process that is referred to as origin licensing. It was often pondered what a cell's fate would be if not all of its replication origins were licensed and subsequently activated during S phase. One obvious prediction was that S phase would simply be prolonged. As it turns out, however, the consequences are much more complex. A short G(1) phase enforced by premature entry into S phase, or other events that negatively affect origin licensing, will ultimately compromise the cell's ability to complete DNA replication before entering mitosis. As a result, the cell becomes genomically unstable when it attempts to repair unreplicated DNA during anaphase. Thus, the density of active replication origins in the chromosomes of eukaryotic cells determines S phase dynamics and chromosome stability during mitosis.