CALPONIN AND SM22 AS DIFFERENTIATION MARKERS OF SMOOTH-MUSCLE - SPATIOTEMPORAL DISTRIBUTION DURING AVIAN EMBRYONIC-DEVELOPMENT
CALPONIN AND SM22 AS DIFFERENTIATION MARKERS OF SMOOTH-MUSCLE - SPATIOTEMPORAL DISTRIBUTION DURING AVIAN EMBRYONIC-DEVELOPMENT
复制标题
DOI：
10.1111/j.1432-0436.1993.tb00027.x
复制
发表时间：
1993-12-01
期刊：
影响因子：
2.9
通讯作者：
SMALL, JV
中科院分区：
文献类型：
--
作者：
DUBAND, JL;GIMONA, M;SMALL, JV
钙调蛋白和SM 22是两种序列相关的蛋白质，在平滑肌细胞中特别丰富。在这里，钙调蛋白和SM 22的分布模式进行了比较与其他平滑肌收缩和细胞骨架成分在禽胚胎中使用免疫荧光显微镜和免疫印迹。像肌球蛋白轻链激酶和重钙调蛋白一样，钙调蛋白和SM 22或多或少只存在于平滑肌细胞中，在胚胎发育期间和成人中。未观察到其他细胞类型（包括横纹肌）的标记。相比之下，原肌球蛋白，平滑肌α-肌动蛋白，细丝蛋白和结蛋白也可以检测到许多其他类型的细胞，除了平滑肌，至少在胚胎生活的一部分。在所有平滑肌细胞群的分化过程中，钙调蛋白和SM 22几乎同步出现，尽管在主动脉的情况下有轻微的时间差。钙调蛋白，SM 22和重caldesmon的外观一般延迟结蛋白，原肌球蛋白，平滑肌α-肌动蛋白，肌球蛋白轻链激酶和细丝蛋白和显着增加，这些蛋白质的丰度观察到在胚胎晚期和成人。从这些观察结果中，我们可以得出结论，钙调蛋白和SM 22属于一组晚期分化的决定因素在平滑肌，并可能构成方便和可靠的标记，以遵循大多数，如果不是所有的，平滑肌细胞群的分化。
Calponin and SM 22 are two proteins related in sequence that are particularly abundant in smooth muscle cells. Here, the distribution patterns of calponin and SM 22 were compared with that of other smooth muscle contractile and cytoskeletal components in the avian embryo using immunofluorescence microscopy and immunoblotting. Like myosin-light-chain kinase and heavy caldesmon, both calponin and SM 22 were more or less exclusively found in smooth muscle cells, during embryonic development and in the adult. Labelling of other cell types including striated muscle was not observed. In contrast, tropomyosin, smooth muscle alpha-actin, filamin and desmin could also be detected in many other cell types in addition to smooth muscles, at least during part of embryonic life. Calponin and SM 22 appeared almost synchronously during the differentiation of all smooth muscle cell populations, though with a slight time difference in the case of the aorta. The appearance of calponin, SM 22 and heavy caldesmon was generally delayed in relation to desmin, tropomyosin, smooth muscle alpha-actin, myosin-light-chain kinase and filamin and a marked increase in abundance of these proteins was observed in the late embryo and in the adult. From these observations we can conclude that both calponin and SM 22 belong to a group of late differentiation determinants in smooth muscle and may constitute convenient and reliable markers to follow the differentiation of most, if not all, smooth muscle cell populations.