Identification of long noncoding RNAs in silkworm larvae infected with Bombyx mori cypovirus.
Identification of long noncoding RNAs in silkworm larvae infected with Bombyx mori cypovirus.
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DOI：
10.1002/arch.21777
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发表时间：
2021-02
影响因子：
2.2
通讯作者：
Zhendong Zhang;Z. Zhao;Su Lin;Wanming Wu;Weiming Tang;Youfu Dong;M. Shen;Ping Wu;Xijie Guo
中科院分区：
文献类型：
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作者：
Zhendong Zhang;Z. Zhao;Su Lin;Wanming Wu;Weiming Tang;Youfu Dong;M. Shen;Ping Wu;Xijie Guo
家蚕细小病毒(Bombyx mori CypoVirus，BmCPV)是引起家蚕严重病害的重要病原之一。越来越多的证据表明，长非编码RNA(LncRNAs)在病毒感染和宿主免疫反应中起着重要的调节作用。为了更好地了解家蚕、家蚕和BmCPV之间的相互作用，我们在感染病毒后的两个时间点对感染病毒的家蚕幼虫中肠的lncRNAs和mRNAs进行了比较转录组分析。共鉴定出16753个基因和1845个候选LncRNAs，其中356个信使RNA(MRNAs)和41个LncRNAs差异表达(DE)。目的基因预测表明，大多数DEmRNAs(123)与28个DElncRNAs共表达，这表明BmCPV诱导的lncRNAs主要通过反式调控影响mRNA的表达，从而构建了DElncRNAs和DEmRNAs的调控网络。根据该网络，许多参与细胞凋亡、自噬和抗病毒反应的基因，如ATG3、PDCD6、IBP2和MFB1，可以被不同的DElncRNAs靶向，这意味着这些基因和lncRNAs在BmCPV感染中起着重要的作用。总之，我们的研究首次揭示了BmCPV感染幼虫中lncRNA表达的变化及其对BmCPV复制的潜在影响，为研究宿主与环状病毒的相互作用提供了新的视角。
Bombyx mori cypovirus (BmCPV) is one of the most important pathogens causing severe disease to silkworm. Emerging evidence indicates that long noncoding RNAs (lncRNAs) play importantly regulatory roles in virus infection and host immune response. To better understand the interaction between silkworm, Bombyx mori and BmCPV, we performed a comparative transcriptome analysis on lncRNAs and mRNAs between the virus-infected and noninfected silkworm larvae midgut at two time points postinoculation. A total of 16,753 genes and 1845 candidate lncRNAs were identified, among which 356 messenger RNA (mRNAs) and 41 lncRNAs were differentially expressed (DE). Target gene prediction revealed that most of DEmRNAs (123) were coexpressed with 28 DElncRNAs, suggesting that the expression of mRNA is mainly affected through trans- regulation by BmCPV-induced lncRNAs, and a regulatory network of DElncRNAs and DEmRNAs was then constructed. According to the network, many genes involved in apoptosis, autophagy, and antiviral response, such as ATG3, PDCD6, IBP2, and MFB1, could be targeted by different DElncRNAs, implying the essential roles of these genes and lncRNAs in BmCPV infection. In all, our studies revealed for the first time the alteration of lncRNA expression in BmCPV-infected larvae and its potential influence on BmCPV replication, providing a new perspective for host-cypovirus interaction studies.