Ultrastructural sequence of myelin breakdown during Wallerian degeneration in the rat optic nerve
Ultrastructural sequence of myelin breakdown during Wallerian degeneration in the rat optic nerve
复制标题
大鼠视神经华勒变性过程中髓磷脂分解的超微结构序列
DOI：
10.1007/bf00214537
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发表时间：
2004
影响因子：
3.6
通讯作者：
C. Shen
中科院分区：
文献类型：
--
作者：
Keh‐Min Liu;C. Shen
对成年白化病大鼠进行单侧眼球手术切除。存活7-140天后，神经胶质细胞的数量反应，以及视神经髓鞘的逐步解体，在电子显微镜蒙太奇定性和定量研究。变性视神经中小胶质细胞和星形胶质细胞的分布密度分别在35和56天后达到高峰，而少突胶质细胞的分布密度逐渐减少。在退化的早期阶段，小胶质细胞出现，并侵入在间期线的鞘，剥离外板，然后被吞噬吞噬。在小胶质细胞内，髓鞘碎片被卷曲的膜包围以形成髓鞘环。在变性的中期，由髓鞘碱性蛋白组成的环状结构的电子致密线分解，形成均一或异质的嗜锇层状结构，即髓鞘体，髓鞘体在变性的后期解体，转化为球状脂滴和针状胆固醇晶体。
Adult albino rats were subjected to unilateral surgical removal of the eyeball. After survival times of 7–140 days, the numerical response of the neuroglial cells, and the progressive disintegration of the myelin sheaths in the optic nerves, were studied qualitatively and quantitatively in electron-microscopic montages. The distribution density of microglia and astroglia in degenerating optic nerve increased to peaks after 35 and 56 days respectively, whereas, the oligodendroglia gradually decreased. During the early stage of degeneration, microglial cells appeared and invaded the sheath at the intraperiod line, peeling off the outer lamellae, which were then engulfed by phagocytosis. Within the microglia, myelin sheath fragments were surrounded by a membrane curled to form a myelin ring. In the intermediate stage of degeneration, the paired electrondense lines of the ring, made up of myelin basic protein, decomposed and formed a homogenous or heterogenous osmiophilic layered structure, the myelin body, which, in the final stages, disintegrated and transformed into globoid lipid droplets and needle shaped cholesterol crystals.