Demasculinisation of sexually mature male common carp, Cyprinus carpio, exposed to 4-tert-pentylphenol during spermatogenesis
Demasculinisation of sexually mature male common carp, Cyprinus carpio, exposed to 4-tert-pentylphenol during spermatogenesis
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DOI：
10.1016/s0166-445x(98)00060-5
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发表时间：
1998-10-01
影响因子：
4.5
通讯作者：
Bowmer, T
中科院分区：
文献类型：
--
作者：
Gimeno, S;Komen, H;Bowmer, T
性成熟的雄性鲤鱼（Cyprinus carpio）在精子发生过程中暴露于亚致死浓度的4-叔戊基苯酚（TPP）和17 β-雌二醇（E2）3个月。这是一个广泛的研究计划的一部分，调查TPP对鲤鱼生命阶段的影响，这可能证明容易受到内分泌干扰。暴露于假雌激素TPP或性成熟过程中暴露于E2，导致成熟雄性鲤鱼血浆中卵黄蛋白原水平升高（P < 0.001），这是一种典型的雌性蛋白质，并抑制精子发生。睾丸的组织学检查显示，在所有TPP和E2浓度下，精子和生精囊肿逐渐消失，并且随着时间的推移，病理学变化的发生率较高（例如，生发上皮纤维化、空泡化和萎缩）。在暴露于所有TPP浓度3个月的鱼睾丸中以及在一些TPP暴露1个月和2个月后，测量到生精小叶直径减小（P < 0.001）。暴露3个月后，暴露于最高TPP浓度的鱼的精子压积值显著低于对照组（P < 0.01），而暴露于最高E2浓度的鱼没有产生任何精液。早期卵睾丸的发生在一些鱼完全退化的睾丸表明，卵子发生可能会进行在睾丸已首先被抑制（demasculinised）后雌激素刺激。(C)1998 Elsevier Science B. V.保留所有权利。
Sexually mature male common carp (Cyprinus carpio) were exposed for a 3-month period to sublethal concentrations of 4-tert-pentylphenol (TPP) and to 17 beta-estradiol (E2) during spermatogenesis. This was part of a broad research programme investigating the effects of TPP on the life stages of the carp which might prove susceptible to endocrine disruption. Exposure of adult male carp to the pseudo-estrogen TPP, or to E2 during sexual maturation, led to elevated plasma levels of vitellogenin (P < 0.001), a typically female protein and to the inhibition of the spermatogenesis. The histological examination of the testes showed the progressive disappearance of spermatozoa and spermatogenic cysts, and a higher incidence of pathological alterations with time at all TPP and E2 concentrations (e.g. fibrosis, vacuolation and atrophy of the germinal epithelium). A reduced diameter of the seminiferous lobules (P < 0.001) was measured in fish testes exposed to all TPP concentrations for 3 months and in some TPP exposures, after 1 and 2 months. After a 3-month exposure, the spermatocrit values of fish exposed to the highest TPP concentration were significantly lower than those of the control (P < 0.01), whilst those fish exposed to the highest E2 concentration did not produce any milt. The occurrence of early ovo-testes in some fish with completely regressed testes suggests that oogenesis may proceed in testes which have first been inhibited (demasculinised) following estrogenic stimulus. (C) 1998 Elsevier Science B.V. All rights reserved.