Sequence of a cDNA from Drosophila coding for the 16 kD proteolipid component of the vacuolar H(+)-ATPase.
Sequence of a cDNA from Drosophila coding for the 16 kD proteolipid component of the vacuolar H(+)-ATPase.
复制标题
来自果蝇的 cDNA 序列，编码液泡 H( )-ATP 酶的 16 kD 蛋白脂质成分。
作者：
L. Meagher;P. Mclean;M. Finbow
液泡H ~+-ATP酶是一类能酸化真核细胞液泡内部的内膜泵。主要的膜成分是分子量为16 kD的蛋白脂，从酵母基因2和牛cDNA 3推导的氨基酸序列显示出高度的一致性，与FIFOH+-ATP合酶的8 kD亚基c蛋白脂的一致性较低。一个相关的，可能相同的16 kD蛋白质也被发现是一个组件的间隙连接样结构分离自动物组织4。我们已经从果蝇中分离出了编码16 kD蛋白脂质的cDNA，作为研究的一部分，以了解这种蛋白质在双层中的结构和分布。牛的cDNA编码的16 kD蛋白脂质首先获得通过筛选商业上可获得的cDNA文库从肾上腺髓质使用寡核苷酸探针来自公布的sequence 3。通过用来自牛cDNA的随机引物探针筛选，从λ gt 10的幼虫文库中鉴定果蝇cDNA。来自果蝇cDNA的最长开放阅读框的推导的氨基酸序列显示出与牛和酵母序列的高度同一性，并且与褐家鼠16 kD蛋白脂质的差距连接形式的直接氨基酸序列高度同一性（Finbow等人，未公布的结果）。我们对果蝇幼虫cDNA文库的恩赐.这项工作是由癌症研究运动。
The vacuolar H +-ATPases are a class of endomembrane pumps which acidify the interiors of vacuolar compartments of eukaryotic cells'. The major membrane component is a proteolipid of MW l6kD and the deduced amino acid sequences from a yeast gene 2 and from a bovine cDNA3 show high identity with each other and a lower identity with the 8kD subunit c proteolipid of the FIFOH+-ATP synthase. A related, possibly identical l6kD protein has also been found to be a component of gap junction-like structures isolated from animal tissues4. We have isolated a cDNA from Drosophila coding for the 16kD proteolipid as part of a study to understand the structure and disposition of this protein in the bilayer. A bovine cDNA coding for the 16kD proteolipid was first obtained by screening a commercially available cDNA library from adrenal medulla using oligonucleotide probes derived from the published sequence3. The Drosophila cDNA was identified from a larval library in lambda gtlO by screening with randomly primed probes from the bovine cDNA. The deduced amino acid sequence of the longest open reading frame from the Drosophila cDNA shows a high degree of identity with the bovine and yeast sequences and with the direct amino acid sequence of the gap junction form of the Nephrops norvegicus l6kD proteolipid (Finbow et al., unpublished results). We to the kind gift of the Drosophila larval cDNA library. This work was by the Cancer Research Campaign.