Liver glucose transporter: a basolateral protein in hepatocytes and intestine and kidney cells.
Liver glucose transporter: a basolateral protein in hepatocytes and intestine and kidney cells.
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DOI：
10.1152/ajpcell.1990.259.2.c279
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发表时间：
1990-07
期刊：
影响因子：
--
通讯作者：
B. Thorens;Z. Cheng;Dennis Brown;H. Lodish
中科院分区：
文献类型：
--
作者：
B. Thorens;Z. Cheng;Dennis Brown;H. Lodish
促进扩散葡萄糖转运蛋白的“肝”亚型主要在肝、肠、肾和胰岛的β细胞中表达。该转运蛋白在肝脏、肾脏和肠道的表观分子质量是不同的，这是通过使用抗肽抗体对膜蛋白进行Western印迹分析所检测到的。然而，Northern印迹分析和分子克隆表明，相同的mRNA在这些组织中表达，表明相同的转运蛋白多肽存在组织特异性的翻译后修饰。冰冻组织切片免疫荧光分析表明，肝葡萄糖转运蛋白存在于肝细胞的肝窦膜、完全分化的吸收肠上皮细胞的基侧膜和肾小管近端小管细胞的基侧膜上。这一定位与肝脏葡萄糖转运蛋白参与葡萄糖代谢的几个关键步骤相一致：肝脏摄取和释放葡萄糖，肠和肾上皮细胞分别吸收或重吸收。
The "liver" isoform of the facilitated diffusion glucose transporter is expressed predominantly in liver, intestine, kidney, and pancreatic islet beta-cells. The apparent molecular mass of the transporter in liver, kidney, and intestine is different, as detected by Western blot analysis of membrane proteins using antipeptide antibodies. However, as assessed by Northern blot analysis and molecular cloning, the same mRNA is expressed in these tissues, indicating that there are tissue-specific posttranslational modifications of the same transporter polypeptide. As determined by immunofluorescence analysis on frozen tissue sections, the liver glucose transporter is present on the sinusoidal membrane of hepatocytes, on the basolateral membrane of fully differentiated absorptive intestine epithelial cells, and on the basolateral membrane of proximal tubule cells of the kidney nephron. This localization is consistent with the involvement of the liver glucose transporter in several key steps of glucose metabolism: glucose uptake and release by the liver and absorption or reabsorption by epithelial cells of the intestine and kidney, respectively.