Fluorescent sensors for Zn2+ based on a fluorescein platform:: Synthesis, properties and intracellular distribution
Fluorescent sensors for Zn2+ based on a fluorescein platform:: Synthesis, properties and intracellular distribution
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DOI：
10.1021/ja010059l
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发表时间：
2001-08-15
影响因子：
15
通讯作者：
Lippard, SJ
中科院分区：
文献类型：
--
作者：
Burdette, SC;Walkup, GK;Lippard, SJ
合成并表征了两种以荧光素为报告基团的新型锌离子荧光传感器Zinpyr-1和Zinpyr-2。Zinpyr-1通过Mannich反应一步制得，Zinpyr-2以4‘，5’-荧光素二甲醛为关键中间体，经多步合成得到。两种Zinpyr传感器的激发和发射波长都在可见光范围内(类似于500 nm)，锌离子的离解常数(K-d1)是
Two new fluorescent sensors for Zn2+ that utilize fluorescein as a reporting group, Zinpyr-1 and Zinpyr-2, have been synthesized and characterized. Zinpyr-1 is prepared in one step via a Mannich reaction, and Zinpyr-2 is obtained in a multistep synthesis that utilizes 4 ' ,5 ' -fluorescein dicarboxaldehyde as a key intermediate. Both Zinpyr sensors have excitation and emission wavelengths in the visible range (similar to 500 nm), dissociation constants (K-d1) for Zn2+ of