Null alleles of SAC7 suppress temperature-sensitive actin mutations in Saccharomyces cerevisiae.
Null alleles of SAC7 suppress temperature-sensitive actin mutations in Saccharomyces cerevisiae.
复制标题
SAC7 的空等位基因抑制酿酒酵母中温度敏感的肌动蛋白突变。
DOI：
10.1128/mcb.10.5.2308-2314.1990
复制
发表时间：
1990
影响因子：
5.3
通讯作者：
Shortle,D
中科院分区：
文献类型：
--
作者：
Dunn,TM;Shortle,D
分离了一个新的温度敏感性突变（actl-4）的基因外抑制子，试图鉴定其产物直接与肌动蛋白相互作用的基因。一个具有冷敏感生长表型的抑制子定义了新基因SAC 7，该基因被定位、克隆、测序和破坏。对SAC 7破坏菌株的遗传分析表明，该蛋白是低温下正常生长和肌动蛋白组装所必需的。SAC 7基因的无效突变也抑制了由act 1- 1和actl-4突变引起的温度敏感性生长缺陷，而与温度敏感性等位基因act 1- 2联合使用则是致死性的。这些结果支持了SAC 7基因产物参与肌动蛋白的正常组装或功能或两者兼而有之的观点。
Extragenic suppressors of a new temperature-sensitive mutation (actl-4) in the actin gene ofSaccharomyces cerevisiaewere isolated in an attempt to identify genes whose products interact directly with actin. One suppressor with a cold-sensitive growth phenotype defined the new gene,SAC7,which was mapped, cloned, sequenced, and disrupted. Genetic analysis of strains that are disrupted forSAC7demonstrated that the protein is required for normal growth and actin assembly at low temperatures. Surprisingly, null mutations inSAC7also suppressed the temperature-sensitive growth defect caused by theact 1-1andactl-4mutations, whereas they were lethal in combination with the temperature-sensitive alleleact 1-2.These results support the notion that theSAC7gene product is involved in the normal assembly or function or both of actin.