Molecular mapping of a dominant non-glaucousness gene from synthetic hexaploid wheat (Triticum aestivum L.)
Molecular mapping of a dominant non-glaucousness gene from synthetic hexaploid wheat (Triticum aestivum L.)
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合成六倍体小麦 (Triticum aestivum L.) 显性非青色基因的分子图谱
DOI：
10.1007/s10681-006-9302-5
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发表时间：
2007-05-01
期刊：
影响因子：
1.9
通讯作者：
Sun, Qixin
中科院分区：
文献类型：
--
作者：
Liu, Qing;Ni, Zhongfu;Sun, Qixin
许多陆生植物的叶和茎表面覆盖着一层表皮蜡层，使其呈现出灰白色或白色花朵。人工合成的六倍体小麦品系3672为非白霜型，普通小麦品系9753为白霜型。使用扫描电子显微镜（SEM）表征表皮蜡。以9753为母本，3672为父本配制杂交组合，对108个F_2代及其F_3代进行了非蓝性基因定位。品系3672的无白度受一对显性基因控制，暂定名为Iw 3672。5个SSR标记定位在2DS染色体上，与Iw 3672连锁。此外，开发了两个EST衍生的标记和一个SNP标记，也与Iw 3672连锁。8个标记与Iw 3672的遗传距离依次为Xte 6730/Xbarc 124520-Iw 3672-Xwe 62100/2150 -Xcau 96287-Xcfd 51180/200/230 -Xwe 7-Xgdm 5190-Xgdm 35246/250，每个区间的遗传距离分别为0.9cM、1.4cM、0.9cM、0.9cM、1.9cM、7.2 cM和2.5 cM。我们的结论是，Iw 3672物理定位在小麦染色体2DS的远端区域。
The leaf and stem surfaces of many land plants are covered with a cuticular wax layer that confers a glaucous appearance or white bloom. Synthetic hexaploid wheat Line 3672 was non-glaucous, and common wheat Line 9753 was glaucous. The cuticular wax was characterized using Scanning Electron Microscopy (SEM). A hybrid using 9753 as female parent and 3672 as male parent was made and 108 F2 plants and their F3 progenies were used to map the non-glaucouness gene. Non-glaucousness in Line 3672 was controlled by a single dominant gene, temporarily designated Iw3672. Five SSR markers mapped on chromosome 2DS were linked to Iw3672. Additionally, two EST-derived markers and a SNP marker were developed and were also linked to Iw3672. The order of the eight markers and Iw3672 was Xte6730 /Xbarc124520 —Iw3672 —Xwe62100/2150 —Xcau96287 —Xcfd51180/200/230 —Xwe7—Xgdm5190 —Xgdm35246/250, with the genetic distance for each interval being 0.9 cM, 1.4 cM, 0.9 cM, 0.9 cM, 1.9 cM, 7.2 cM and 2.5 cM, respectively. We concluded that Iw3672 is physically mapped on the distal region of wheat chromosome 2DS.