AN ELECTROPHORETICALLY DETECTABLE MODIFICATION OF GLUCOSEPHOSPHATE ISOMERASE IN MOUSE SPERMATOZOA
AN ELECTROPHORETICALLY DETECTABLE MODIFICATION OF GLUCOSEPHOSPHATE ISOMERASE IN MOUSE SPERMATOZOA
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DOI：
10.1530/jrf.0.0630169
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
MCLAREN, A
中科院分区：
文献类型：
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作者：
BUEHR, M;MCLAREN, A
小鼠睾丸和精子的葡萄糖磷酸异构酶(GPI)的醋酸纤维素电泳谱显示有一条其他组织的GPI未见的小条带。次要条带与所检测的两种GPI的电泳型变异体相同。用不同的酶、酶抑制剂或硫醇试剂处理组织提取物，不能改变精子和睾丸GPI中次要条带的出现或位置，也不能诱导其他组织的GPI中出现这些条带。在未成熟男性或因遗传原因睾丸缺乏精子的男性的GPI电泳图中，未见次要条带。
Cellulose acetate electrophoresis of glucose phosphate isomerase (GPI) from mouse testis and spermatozoa revealed a minor band which is not seen in electropherograms of GPI from other tissues. The minor band is associated equally with the 2 electrophoretic variants of GPI examined. Treatment of tissue extracts with various enzymes, enzyme inhibitors or thiol reagents failed to alter the appearance or position of the minor bands in sperm and testis GPI, or to induce their appearance in GPI from other tissues. No minor bands were seen in electropherograms of testis GPI from immature males, or from males whose testes lack spermatozoa for genetic reasons.