Lab-on-a-chip platforms for quantification of multicellular interactions in bone remodeling
Lab-on-a-chip platforms for quantification of multicellular interactions in bone remodeling
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DOI：
10.1016/j.yexcr.2018.02.027
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发表时间：
2018-04-01
影响因子：
3.7
通讯作者：
Saunders, Marnie M.
中科院分区：
文献类型：
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作者：
George, Estee L.;Truesdell, Sharon L.;Saunders, Marnie M.
研究人员一直在使用芯片实验室系统来分离研究因素，模拟实验室分析并模拟细胞，组织和器官水平的过程。这项技术正在迅速发展，但骨领域的发展速度却很慢。需要新的模型，有能力和灵活性，调查优雅和同步的多细胞相互作用，发生在正常的骨转换和疾病状态，其中涉及重塑。通过消除时间和空间限制并实现功能结果的量化，平台应该提供比单细胞类型系统更仿生的独特环境，同时最大限度地减少体内模型的复杂全身效应。这份手稿详细介绍了用于刺激骨细胞和量化骨重建的芯片实验室平台的开发和表征。我们的平台为模型提供了基础，该模型可用于整体研究重塑相互作用，或作为标准的机械转导工具，通过该工具，可以将孤立的活动量化为负荷的函数。
Researchers have been using lab-on-a-chip systems to isolate factors for study, simulate laboratory analysis and model cellular, tissue and organ level processes. The technology is increasing rapidly, but the bone field has been slow to keep pace. Novel models are needed that have the power and flexibility to investigate the elegant and synchronous multicellular interactions that occur in normal bone turnover and in disease states in which remodeling is implicated. By removing temporal and spatial limitations and enabling quantification of functional outcomes, the platforms should provide unique environments that are more biomimetic than single cell type systems while minimizing complex systemic effects of in vivo models. This manuscript details the development and characterization of lab-on-a-chip platforms for stimulating osteocytes and quantifying bone remodeling. Our platforms provide the foundation for a model that can be used to investigate remodeling interactions as a whole or as a standard mechanotransduction tool by which isolated activity can be quantified as a function of load.