Protective effect of silymarin on erythrocyte haemolysate against benzo(a)pyrene and exogenous reactive oxygen species (H2O2) induced oxidative stress
Protective effect of silymarin on erythrocyte haemolysate against benzo(a)pyrene and exogenous reactive oxygen species (H2O2) induced oxidative stress
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DOI：
10.1016/j.chemosphere.2007.03.015
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发表时间：
2007-07-01
期刊：
影响因子：
8.8
通讯作者：
Devi, K. Pandima
中科院分区：
文献类型：
--
作者：
Kiruthiga, P. V.;Shafreen, R. Beema;Devi, K. Pandima
本研究旨在评价水飞蓟素（水飞蓟）在人红细胞溶血产物中对苯并（α）芘[B（α）PI，一种强致癌化学物质的体外抗氧化特性和保护作用。通过监测三组溶血产物中的抗氧化酶和丙二醛来评估水飞蓟素的体外保护作用：（I）载体对照、（II）B（α）P孵育组和（III）B（α）P与水飞蓟素共孵育。在溶血产物中评估了水飞蓟素对脂质过氧化（LPO）和抗氧化酶[超氧化物歧化酶; SOD，过氧化氢酶; CAT，谷胱甘肽过氧化物酶; GPx，谷胱甘肽还原酶; GR和谷胱甘肽-S-转移酶; GST]的影响。有人观察到，抗氧化酶的比活性显着降低，丙二醛水平升高时，溶血产物与B（a）P.水飞蓟素的保护作用阐明了显着逆转的抗氧化酶和降低丙二醛的水平。此外，将溶血产物与B（α）P孵育45 min，并使用HPLC检测B（α）P代谢物3-羟基苯并（α）芘（3-OH-B（α）P）。在第II组中检测到代谢物水平升高。然而，当溶血产物与水飞蓟素共孵育时，未检测到反应性代谢物3-OH-B（a）P，这进一步证实了水飞蓟素的保护作用。II组中3-OH-B（a）P的产生暗示了在细胞色素P4501 A1（CYP 1A 1）介导的I相代谢过程中溶血产物中产生活性氧（O-2（-）和H2 O2）的可能性。因此，我们将溶血液与外源性活性氧H2 O2一起孵育，并评估了水飞蓟素对H2 O2的保护作用。结果表明，水飞蓟素对环境污染物引起的氧化应激损伤具有明显的保护作用和清除自由基的机制。(C)2007爱思唯尔有限公司保留所有权利。
The present study was carried out to evaluate the in vitro antioxidant properties and protective effects of silymarin (milk thistle) in human erythrocyte haemolysates against benzo(alpha)pyrene [B(alpha)PI, a potent carcinogenic chemical. Protective effect of silymarin was assessed in vitro by monitoring the antioxidant enzymes and malondialdehyde in three groups of haemolysates-(I) vehicle control (II) B(alpha)P incubated group and (III) B(alpha)P co incubated with silymarin. The effects of silymarin on lipid peroxidation (LPO) and antioxidant enzymes [superoxide dismutase; SOD, catalase; CAT, glutathione peroxidase; GPx, glutathione reductase; GR and glutathione-S-transferases; GST] were assessed on haemolysates. It was observed that specific activity of antioxidant enzymes were significantly decreased and the malondialdehyde levels were elevated when haemolysates were incubated with B(a)P. The protective effect of silymarin is elucidated by the significant reversal of the antioxidant enzymes and reduction in the levels of malondialdehyde. In addition, haemolysates were incubated with B(alpha)P for 45 min and the B(alpha)P metabolite, 3-hydroxy benzo(alpha)pyrene (3-OH-B(alpha)P) was detected using HPLC. An increased level of the metabolite was detected in group II. Whereas, when haemolysates were co-incubated with silymarin, the reactive metabolite 3-OH-B(a)P was not detectable which further confirms the protective role of silymarin. Generation of 3-OH-B(a)P in group II implicates the possibility of reactive oxygen species (O-2(-) and H2O2) production in haemolysates during cytochrome P4501A1 (CYP1A1) mediated Phase-I-metabolism. Hence, we incubated the haemolysates with exogenous reactive oxygen species H2O2 and assessed the protective role of silymarin against H2O2. From the results of our study, it was suggested that silymarin possess substantial protective effect and free radical scavenging mechanism against environmental contaminants induced oxidative stress damages. (C) 2007 Elsevier Ltd. All rights reserved.