Accurate quantification of brown adipose tissue mass by xenon-enhanced computed tomography
Accurate quantification of brown adipose tissue mass by xenon-enhanced computed tomography
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DOI：
10.1073/pnas.1714431115
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发表时间：
2018-01-02
影响因子：
11.1
通讯作者：
Burant, Alex
中科院分区：
文献类型：
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作者：
Branca, Rosa T.;McCallister, Andrew;Burant, Alex
棕色脂肪组织（BAT）质量的检测和定量仍然是一个主要的挑战，因为目前的断层成像技术要么是非特异性的，要么缺乏必要的分辨率来量化BAT质量，特别是在肥胖表型，其中该组织可能存在，但不活跃。在这里，我们报告定量BAT质量氙增强计算机断层扫描。我们发现，在刺激BAT产热过程中，亲脂性气体氙优先在BAT中积累，导致放射密度增强与肺中所见相当。这种增强是由BAT血管阻力的选择性降低介导的，这大大增加了BAT的血管灌注。这种增强不仅能够精确识别和量化瘦小鼠表型中的BAT质量，而且还能够精确识别和量化肥胖小鼠表型中的BAT质量，其中该组织对于传统的断层成像技术是不可见的。该方法在啮齿动物中开发和验证，然后应用于猕猴，以评估其在较大物种中的可行性。
Detection and quantification of brown adipose tissue (BAT) mass remains a major challenge, as current tomographic imaging techniques are either nonspecific or lack the necessary resolution to quantify BAT mass, especially in obese phenotypes, in which this tissue may be present but inactive. Here, we report quantification of BAT mass by xenon-enhanced computed tomography. We show that, during stimulation of BAT thermogenesis, the lipophilic gas xenon preferentially accumulates in BAT, leading to a radiodensity enhancement comparable to that seen in the lungs. This enhancement is mediated by a selective reduction in BAT vascular resistance, which greatly increases vascular perfusion of BAT. This enhancement enables precise identification and quantification of BAT mass not only in lean, but also in obese, mouse phenotypes, in which this tissue is invisible to conventional tomographic imaging techniques. The method is developed and validated in rodents and then applied in macaques to assess its feasibility in larger species.