Mobile teledermatology for skin tumour screening: diagnostic accuracy of clinical and dermoscopic image tele-evaluation using cellular phones
Mobile teledermatology for skin tumour screening: diagnostic accuracy of clinical and dermoscopic image tele-evaluation using cellular phones
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DOI：
10.1111/j.1365-2133.2011.10208.x
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发表时间：
2011-05-01
影响因子：
10.3
通讯作者：
Hofmann-Wellenhof, R.
中科院分区：
文献类型：
--
作者：
Kroemer, S.;Fruehauf, J.;Hofmann-Wellenhof, R.
背景皮肤的屏障功能可以通过多种生物物理和分子方法来表征。屏障特性随深度变化的变化尚未得到详细探讨。 目的 表征前臂腹侧角质细胞表面积、角质细胞成熟度、选定蛋白酶活性和经皮失水 (TEWL) 随深度增加的变化。方法选择 22 名健康志愿者的左中腹前臂作为研究部位。胶带剥离后，评估角质细胞成熟度和表面积。使用荧光探针测定法测量脱屑激肽释放酶、KLK5 和 KLK7 以及炎性类胰蛋白酶的蛋白酶活性。还记录了蛋白质含量和 TEWL。 结果随着胶带剥离次数（即进入皮肤的深度）的增加，角质细胞成熟度和表面积降低。较成熟的角质细胞通常比较不太成熟的角质细胞更大。脱屑酶和炎症酶的蛋白酶活性以及蛋白质含量在角质层外层最高，并随着深度而降低。正如预期的那样，随着更多角质层被去除，TEWL 增加。对于所研究的所有参数，男性和女性、白人和黑人受试者之间没有统计学差异。结论本研究中使用的技术提供了角质层不同级别内角质细胞成熟度和表面积的空间分布以及蛋白酶活性和蛋白质含量的快速无创测量。所使用的方法将有助于深入了解配方赋形剂和活性成分对表皮更新和皮肤屏障功能的影响。
P>BackgroundThe barrier function of the skin may be characterized by a number of biophysical and molecular methods. Variation in the barrier properties as a function of depth has not been explored in detail.ObjectivesTo characterize changes in corneocyte surface area, corneocyte maturity, selected protease activities and transepidermal water loss (TEWL) in the ventral forearm with increasing depth.MethodsThe left mid-ventral forearm of 22 healthy volunteers was selected as the study site. After tape stripping, corneocyte maturity and surface area were assessed. The protease activity of the desquamatory kallikrein proteases, KLK5 and KLK7, and inflammatory tryptase was measured using a fluorogenic probe assay. Protein content and TEWL were also recorded.ResultsCorneocyte maturity and surface area decreased with increasing number of tape strippings, i.e. depth into the skin. More mature corneocytes were typically larger than less mature corneocytes. The protease activities of both the desquamatory and inflammatory enzymes together with the protein content were highest in the outer layers of the stratum corneum and decreased with depth. As expected, TEWL increased as more stratum corneum layers were removed. There were no statistical differences between men and women or caucasian and black subjects for all of the parameters studied.ConclusionsThe techniques used in this study provide rapid noninvasive measures of the spatial distribution of corneocyte maturity and surface area as well as protease activity and protein content within different levels of the stratum corneum layers. The methods used will allow mechanistic insight into the effects of formulation excipients and active ingredients on epidermal turnover and skin barrier function.