Genetic mapping of a wide spectrum nematode resistance gene (Hero) against Globodera rostochiensis in tomato.
Genetic mapping of a wide spectrum nematode resistance gene (Hero) against Globodera rostochiensis in tomato.
复制标题
番茄广谱线虫抗性基因 (Hero) 的遗传图谱。
DOI：
10.1094/mpmi-8-0886
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
Amar Kumar
中科院分区：
文献类型：
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作者：
Martin W. Ganal;R. Simon;S. Brommonschenkel;M. Arndt;Mark S. Phillips;Tanksley Sd;Amar Kumar
Hero 基因赋予对马铃薯胞囊线虫 Globodera rostochiensis 多种致病型的抗性。该基因已从野生番茄品种 Lycopersicon pimpinellifolium 渗入到栽培番茄中。我们使用 RFLP 和 RAPD 分析对栽培番茄中的 L. pimpinellifolium 进行有针对性的搜索。我们使用 RFLP 和 RAPD 分析对 L. pimpinellifolium 片段进行有针对性的搜索。抗性品系 LA 1792 在 4 号染色体上含有单个渐渗片段，其特征在于来自番茄高密度 RFLP 图谱的三个 RFLP 标记。 Hero 基因在大群体中的图谱位置，在基因渗入区域中鉴定出了四个额外的标记。在分析了 800 多个配子的重组情况后，我们发现一个标记距离 Hero 基因仅 0.4 cM。从 Hero 基因附近的区域分离的 YAC 克隆表明，在基因组的该区域，kb/cM 比率相对较低（<450 kb/cM），并且为了分离该基因，染色体行走应该是可行的。
The Hero gene confers resistance to a wide spectrum of pathotypes of the potato cyst nematode Globodera rostochiensis. This gene has been introgressed from the wild tomato species Lycopersicon pimpinellifolium into the cultivated tomato. We have used RFLP and RAPD analysis for the targeted search of the L. pimpinellifolium into the cultivated tomato. We have used RFLP and RAPD analysis for the targeted search of the L. pimpinellifolium segment. The resistant line LA 1792 contains a single introgressed segment on chromosome 4, which is characterized by three RFLP markers from the high-density RFLP map of tomato. The map position of the Hero gene in large populations, four additional markers were identified in the introgressed region. After analyzing more than 800 gametes for recombination, we found that one marker is only 0.4 cM away from the Hero gene. YAC clones isolated from a region near the Hero gene indicate that in this area of the genome, the kb/cM ratio is relatively low (<450 kb/cM) and chromosome walking should be feasible in order to isolate this gene.