Chemotactic factor causes rapid decreases in phosphatidylinositol,4,5-bisphosphate and phosphatidylinositol 4-monophosphate in rabbit neutrophils.
Chemotactic factor causes rapid decreases in phosphatidylinositol,4,5-bisphosphate and phosphatidylinositol 4-monophosphate in rabbit neutrophils.
复制标题
趋化因子导致兔中性粒细胞中磷脂酰肌醇、4,5-二磷酸和磷脂酰肌醇4-单磷酸快速减少。
DOI：
10.1016/0006-291x(83)91711-4
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
Sha'afi,RI
中科院分区：
文献类型：
--
作者：
Volpi,M;Yassin,R;Naccache,PH;Sha'afi,RI
用合成的趋化肽f-Met-Leu-Phe刺激~（32）P预标记的兔中性粒细胞，可使4，5-二磷酸磷脂酰肌醇和4-单磷酸磷脂酰肌醇的放射性迅速降低。刺激后10秒，4，5-二磷酸磷脂酰肌醇的最大降低平均值的平均值±标准误差等于对照值的19 ± 3%。4-单磷酸磷脂酰肌醇的相应值出现在刺激后60秒，等于对照值的37 ± 7%。另一方面，磷脂酸和溶血磷脂的放射性随刺激后时间的推移而持续增加。这些变化与钙释放和中性粒细胞活化的关系进行了讨论。
Stimulation of rabbit neutrophils prelabeled with32P by the synthetic chemotactic peptide f-Met-Leu-Phe induces a rapid decrease in the radio-activity in both phosphatidylinositol, 4,5 bis phosphate and phosphatidylinositol 4-monophosphate. The mean ± standard error of the mean values of the maximum decrease in phosphatidylinositol, 4,5 bis phosphate occurred at 10 seconds following stimulation and is equal to 19 ± 3% of the control value. The corresponding value for phosphatidylinositol 4-monophosphate occurred at 60 seconds following stimulation and is equal to 37 ± 7% of the control value. On the other hand, the radio-activity in phosphatidic acid and lysophospholipids increased continuously with time following stimulation. The relationship of these changes to calcium release and neutrophil activation is discussed.