Enhanced lipid production by Rhodotorula glutinis CGMCC 2.703 using a two-stage pH regulation strategy with acetate as the substrate
Enhanced lipid production by Rhodotorula glutinis CGMCC 2.703 using a two-stage pH regulation strategy with acetate as the substrate
复制标题
使用以乙酸盐为底物的两阶段 pH 调节策略，提高粘红酵母 CGMCC 2.703 的脂质产量
DOI：
10.1002/ese3.413
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发表时间：
2019
影响因子：
3.8
通讯作者：
Zhang Jian An
中科院分区：
文献类型：
--
作者：
Zhang Wen;Wu Jing;Zhou Yu Jie;Liu Hong Juan;Zhang Jian An
近年来，挥发性脂肪酸作为一种新型、廉价的生物柴油生产底物已成为研究热点。研究了pH对红酵母以乙酸盐为底物产脂的影响。最佳乙酸盐初始浓度为23 g/L，C/N为350。在含有23 g/L初始乙酸盐浓度的1 L生物反应器中，细胞生长的最适宜pH为6.6，而发现pH 7.0对于脂质生产是最佳的。在pH恒定补料分批发酵中，在pH 6.6下获得67.2 g/L的增加的生物质产率，而在pH 7.0下获得29.3 g/L的增加的脂质滴度。当使用两阶段pH调节培养策略时，生物量产量、脂质滴度和脂质含量分别达到68.2 g/L、35.8 g/L和52.5%。生物产量和油脂产量均得到明显提高。与分批培养相比，采用两阶段pH调节培养策略，生物量产量、脂质滴度和脂质含量分别提高了15.6倍、19.1倍和21.0%。比较了以乙酸盐和葡萄糖为底物时主要脂肪酸的组成。使用这两种底物产生的脂质的组成与棕榈油的组成相似，表明在乙酸盐存在下由红曲霉产生的脂质显示出用作生物柴油原料的前景。
In recent years, VFAs as a novel, inexpensive substrate for biodiesel production have become a hot research topic. The effect of pH on lipid production byRhodotorula glutiniswhen using acetate as a substrate was studied. The optimized initial acetate concentration and C/N ratio were 23 g/L and 350, respectively. In 1‐L bioreactors containing an initial acetate concentration of 23 g/L, the most suitable pH for cell growth was 6.6, while pH 7.0 was found optimal for lipid production. In the pH‐stat fed‐batch fermentation, an increased biomass yield of 67.2 g/L was obtained at pH 6.6, while an increased lipid titer of 29.3 g/L was obtained at pH 7.0. When using a two‐stage pH regulation culture strategy, the biomass yield, lipid titer, and lipid content reached 68.2 g/L, 35.8 g/L, and 52.5%, respectively. Both the biomass yield and lipid production were obviously enhanced. Compared with the batch culture, the biomass yield, lipid titer, and lipid content were increased 15.6‐fold, 19.1‐fold, and 21.0%, respectively, by using the two‐stage pH regulation culture strategy. The composition of the main fatty acids was compared when acetate or glucose was used as the substrate. The composition of the lipids produced using both substrates was similar to that of palm oil, indicating that lipids produced byR. glutinisin the presence of acetate showed promise for use as a biodiesel feedstock.