A fibrin‐coated pericardial extracellular matrix prevented heart adhesion in a rat model
A fibrin‐coated pericardial extracellular matrix prevented heart adhesion in a rat model
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纤维蛋白包被的心包细胞外基质可预防大鼠模型中的心脏粘连
DOI：
10.1002/jbm.b.34201
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Negishi Jun
中科院分区：
文献类型：
--
作者：
Funamoto Seiichi;Hashimoto Yoshihide;Kishida Akio;Negishi Jun
由于大多数手术治疗都会带来组织粘连的风险，因此各个手术领域都需要防止粘连的方法。在这项研究中，我们研究了使用带有纤维蛋白胶的脱细胞心包来防止大鼠心脏粘连。采用高静水压法对猪心包进行脱细胞处理。在体外细胞接种试验中，细胞粘附到所得的心包细胞外基质（ECM）上，但纤维蛋白包被的心包 ECM 显示细胞粘附减少。在大鼠心脏粘连手术模型中，纤维蛋白包被的心包 ECM 未粘附到心脏，并且在 ECM 表面观察到间皮细胞粘连。值得注意的是，术后 4 周观察到纤维蛋白涂层心包 ECM 的抗粘连作用。这些结果支持纤维蛋白涂层心包ECM作为心血管手术的防粘连材料的实用性。 © 2018 Wiley periodicals, Inc. J Biomed Mater Res B 部分：Appl Biomater 107B：1088–1094，2019。
As most surgical treatments pose a risk of tissue adhesion, methods to prevent adhesion are needed across various surgical fields. In this study, we investigated the use of a decellularized pericardium with fibrin glue to prevent rat heart adhesion. Porcine pericardia were decellularized by a high‐hydrostatic pressure method. Cells adhered to the resulting pericardial extracellular matrix (ECM) during anin vitrocell‐seeding test, but fibrin‐coated pericardial ECM showed reduced cell adhesion. In a rat surgical model of heart adhesion, the fibrin‐coated pericardial ECM did not adhere to the heart and mesothelial cell adhesion was observed on the ECM surface. Notably, the anti‐adhesion effect of fibrin‐coated pericardial ECM was observed 4 weeks after surgery. These results support the utility of fibrin‐coated pericardial ECM as an adhesion prevention material for cardiovascular surgery. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1088–1094, 2019.