Nitric oxide regulates cell behavior on an interactive cell-derived extracellular matrix scaffold.
Nitric oxide regulates cell behavior on an interactive cell-derived extracellular matrix scaffold.
复制标题
一氧化氮调节互动细胞衍生的细胞外基质支架上的细胞行为。
DOI：
10.1002/jbm.a.35524
复制
发表时间：
2015-12
期刊：
影响因子：
--
通讯作者：
Zhao F
中科院分区：
文献类型：
--
作者：
Xing Q;Zhang L;Redman T;Qi S;Zhao F
在组织损伤和创面愈合过程中，细胞、细胞外基质（extracellular matrix， ECM）和介导分子之间存在动态的相互作用，对组织功能修复至关重要。一氧化氮（NO）是积极调节伤口愈合过程中各种生物活性的关键介导分子之一。各种ECM成分作为细胞和中介分子的结合位点，相互作用进一步刺激细胞活动。人间充质干细胞（hMSCs）可以迁移到伤口部位，并通过分化和旁分泌信号传导促进组织再生。这项工作的目的是研究在一个相互作用的富含ecm的微环境中NO对hMSCs的调节作用。为了模拟体内创面基质环境，制备了一种细胞源性ECM支架，该支架能够在体内创面液NO水平范围内释放NO。结果表明，ECM支架释放的微摩尔水平NO对hMSCs的细胞活性有抑制作用。NO损害细胞生长，改变细胞形态，破坏F-actin组织，降低局灶黏附相关分子整合素α5和paxillin的表达。这些结果可能有助于阐明NO在伤口愈合过程中如何作用于hMSCs。
During tissue injury and wound healing process, there are dynamic reciprocal interactions among cells, extracellular matrix (ECM) and mediating molecules which are crucial for functional tissue repair. Nitric oxide (NO) is one of the key mediating molecules that can positively regulate various biological activities involved in wound healing. Various ECM components serve as binding sites for cells and mediating molecules, and the interactions further stimulate cellular activities. Human mesenchymal stem cells (hMSCs) can migrate to the wound site and contribute to tissue regeneration through differentiation and paracrine signaling. The objective of this work was to investigate the regulatory effect of NO on hMSCs in an interactive ECM-rich microenvironment. In order to mimic the in vivo stromal environment in wound site, a cell-derived ECM scaffold that was able to release NO within the range of in vivo wound fluid NO level was fabricated. Results showed that the micro-molar level of NO released from the ECM scaffold had an inhibitory effect on cellular activities of hMSCs. The NO impaired cell growth, altered cell morphology, disrupted the F-actin organization, also decreased the expression of focal adhesion related molecules integrin α5 and paxillin. These results may contribute to the elucidation of how NO acts on hMSCs in wound healing process.