Technologies That Enable Accurate and Precise Nano- to Milliliter-Scale Liquid Dispensing of Aqueous Reagents Using Acoustic Droplet Ejection
Technologies That Enable Accurate and Precise Nano- to Milliliter-Scale Liquid Dispensing of Aqueous Reagents Using Acoustic Droplet Ejection
复制标题
利用声学液滴喷射实现水性试剂的纳米级至毫升级液体分配的技术
DOI：
10.1177/2211068215602191
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Sammy S. Datwani
中科院分区：
文献类型：
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作者：
E. Sackmann;L. Majlof;Annett Hahn;Brent Eaton;Temo Bandzava;J. Daulton;A. Vandenbroucke;Matthew Mock;R. Stearns;S. Hinkson;Sammy S. Datwani
声学液体处理使用聚焦在流体表面的高频声学信号，以高精度和精确度喷射液滴，用于各种生命科学应用。在这里，我们提出了一个多孔源板，回声合格的水库（ER），它可以声学传输超过2.5毫升的流体每井在25-nL的增量使用回声525液体处理器。我们展示了两个Labcyte技术动态流体分析（DFA）的方法和高压（HV）网格，需要保持准确和精确的流体传输从ER在这个体积规模。采用DFA方法动态评估流体的能量需求，并调整声喷射参数以保持恒定速度的液滴。此外，我们表明，HV网格提高液滴速度和聚结在目的地板。这些技术使每个目标孔5微升转移到384孔板，准确度和精密度值优于4%。最后，我们使用ER和Echo 525液体处理器进行定量聚合酶链反应（qPCR）分析，以证明ER的灵活性和更大容量能力的应用。
Acoustic liquid handling uses high-frequency acoustic signals that are focused on the surface of a fluid to eject droplets with high accuracy and precision for various life science applications. Here we present a multiwell source plate, the Echo Qualified Reservoir (ER), which can acoustically transfer over 2.5 mL of fluid per well in 25-nL increments using an Echo 525 liquid handler. We demonstrate two Labcyte technologies—Dynamic Fluid Analysis (DFA) methods and a high-voltage (HV) grid—that are required to maintain accurate and precise fluid transfers from the ER at this volume scale. DFA methods were employed to dynamically assess the energy requirements of the fluid and adjust the acoustic ejection parameters to maintain a constant velocity droplet. Furthermore, we demonstrate that the HV grid enhances droplet velocity and coalescence at the destination plate. These technologies enabled 5-µL per destination well transfers to a 384-well plate, with accuracy and precision values better than 4%. Last, we used the ER and Echo 525 liquid handler to perform a quantitative polymerase chain reaction (qPCR) assay to demonstrate an application that benefits from the flexibility and larger volume capabilities of the ER.