Water-soluble quantum dots for multiphoton fluorescence imaging in vivo
Water-soluble quantum dots for multiphoton fluorescence imaging in vivo
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DOI：
10.1126/science.1083780
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发表时间：
2003-05-30
期刊：
影响因子：
56.9
通讯作者：
Webb, WW
中科院分区：
文献类型：
--
作者：
Larson, DR;Zipfel, WR;Webb, WW
半导体纳米晶体（量子点）用作多光子显微镜的荧光标签，可以在苛刻的生物学环境（例如生物组织）中进行多色成像。我们表征了水溶性的硒化 - 硒化锌硫化物量子点，用于活动物中的多光子成像。这些荧光探针的两光子作用横截面高达47,000个Goeppert-Mayer单元，这是迄今为止多光子显微镜中使用的任何标签中最大的。我们通过活小鼠的皮肤动态地观察到量子点，在毛细血管中数百微米深。我们没有发现在纳秒至毫秒尺度上溶液中闪烁（荧光内际分）的证据。
The use of semiconductor nanocrystals (quantum dots) as fluorescent labels for multiphoton microscopy enables multicolor imaging in demanding biological environments such as living tissue. We characterized water-soluble cadmium selenide-zinc sulfide quantum dots for multiphoton imaging in live animals. These fluorescent probes have two-photon action cross sections as high as 47,000 Goeppert-Mayer units, by far the largest of any label used in multiphoton microscopy. We visualized quantum dots dynamically through the skin of living mice, in capillaries hundreds of micrometers deep. We found no evidence of blinking (fluorescence intermittency) in solution on nanosecond to millisecond time scales.